Protein Info for EX31_RS05875 in Rahnella sp. WP5

Annotation: autotransporter domain-containing esterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 668 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF00657: Lipase_GDSL" amino acids 34 to 348 (315 residues), 65.7 bits, see alignment E=7.1e-22 PF03797: Autotransporter" amino acids 414 to 645 (232 residues), 139.8 bits, see alignment E=1.4e-44

Best Hits

KEGG orthology group: K12686, outer membrane lipase/esterase (inferred from 99% identity to rah:Rahaq_0024)

Predicted SEED Role

"Outer membrane esterase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (668 amino acids)

>EX31_RS05875 autotransporter domain-containing esterase (Rahnella sp. WP5)
MAGKNSSILRLSQGAFAVALCLGLQAPAKAFDQLYVFGDSLSDTGNIGRWTFDSSTHPLY
DEILANNIGTTLAPSSKGGLNYAEGGGVAVPGLDSDYTTQDQVKSYLTSTGGKADPNGLY
IHWIGGNDLAAAALSLNPVTAVEIATNSATQAASQVNDLLKAGAGTVVVPTVPNIGLTPV
LMEGVIQVGLLPVQQQALAAAYQYLNAQTTPDADARTEAIHNALAAAAAEGSGIPLVQDV
IAKGLAAAYDALKDVAGGLTDTYNTTEDIALAKTGGNIARVDINGLFNEVVADPARYGFA
NTAGTACPVGTSASECTSNLPGFDSSKAFLFSDHFHPTPQAHALIAQYMQSVLDGPLEAS
ALSQGSLALARNTQGLLDSRYQQLRTQNNDAGTFGMFGGYAGQSADSKFSPSMGLGDGDL
TANSLNIGMDYQMTDRWMMGLMLSGSDDKDDPTDHYSYRTRGWMATAFTGIKIAENGWIN
GDVHYGSLNYDDIKRRITLGPTTRTERGETDGKQIGGRVTAGWDFPILPVLKTGPVAQYS
WDYSSVDGYSEKGNNSTSMRFGDQTSHSQIGALGWRVNAELGIVNPYAQVTYDRQFGDKT
YTTTGAIKSTRTRFSRTTGEQDDDWVDMTAGLNVQITHSISAFAAISQTTGLDSGEQTSY
NAGLSARF