Protein Info for EX31_RS05410 in Rahnella sp. WP5

Annotation: MHS family MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 transmembrane" amino acids 25 to 53 (29 residues), see Phobius details amino acids 65 to 86 (22 residues), see Phobius details amino acids 97 to 119 (23 residues), see Phobius details amino acids 121 to 121 (1 residues), see Phobius details amino acids 123 to 150 (28 residues), see Phobius details amino acids 169 to 189 (21 residues), see Phobius details amino acids 198 to 218 (21 residues), see Phobius details amino acids 251 to 271 (21 residues), see Phobius details amino acids 291 to 310 (20 residues), see Phobius details amino acids 322 to 340 (19 residues), see Phobius details amino acids 346 to 370 (25 residues), see Phobius details amino acids 385 to 405 (21 residues), see Phobius details amino acids 411 to 431 (21 residues), see Phobius details PF00083: Sugar_tr" amino acids 24 to 227 (204 residues), 85.1 bits, see alignment E=5e-28 amino acids 279 to 440 (162 residues), 45 bits, see alignment E=7.3e-16 PF07690: MFS_1" amino acids 30 to 382 (353 residues), 100.4 bits, see alignment E=1.1e-32 amino acids 284 to 431 (148 residues), 56.9 bits, see alignment E=1.8e-19 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 31 to 426 (396 residues), 492.9 bits, see alignment E=3.7e-152

Best Hits

Swiss-Prot: 80% identical to YHJE_ECOLI: Inner membrane metabolite transport protein YhjE (yhjE) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to rah:Rahaq_0117)

Predicted SEED Role

"Inner membrane metabolite transport protein YhjE"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (440 amino acids)

>EX31_RS05410 MHS family MFS transporter (Rahnella sp. WP5)
MQATITPELEADAQLVSQNSRSKVIVASLIGTAIEFFDFYIYATAAVIVFPHIFFPQGDP
TAATLQSLATFAIAFVARPIGSALFGHFGDRVGRKVTLVASLLTMGVSTVVIGLLPTYAT
IGVFAPMLLALARFGQGLGLGGEWGGAALLATENAPAKKRALYGSFPQLGAPIGFFFANG
MFLLLSWLLTDEQFISWGWRVPFLLSAVLVIVGLYVRVSLHETPVFAKIAKAGKQVKVPI
GTLFTKYKKATILGTFIMVATYTLFYIMTVYSLGYGTAAKPVGLGIPRNEFLLMLMIGVV
GFGVMVPVAGQLADKFGRRKTMICITLLMIVFALCFPTILGAGSQALVMVFLLCGFMVMG
LTFGPMGALLPELFPTEVRYTGASFSYNVSSILGASVAPYIATWLATHYGLFYVGVYLAS
MATLTLIALLLSKETAHQSL