Protein Info for EX31_RS05365 in Rahnella sp. WP5

Annotation: malate:quinone oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01320: malate dehydrogenase (acceptor)
  • amino acids 28 to 513 (486 residues), 705.6 bits, E=1.6e-216
• PF06039: Mqo
  • amino acids 28 to 515 (488 residues), 804.2 bits, E=3.8e-246
• PF01266: DAO
  • amino acids 29 to 301 (273 residues), 29.8 bits, E=4.5e-11

Best Hits

Swiss-Prot: 78% identical to MQO_SERP5: Probable malate:quinone oxidoreductase (mqo) from Serratia proteamaculans (strain 568)

KEGG orthology group: K00116, malate dehydrogenase (quinone) [EC: 1.1.5.4] (inferred from 100% identity to rah:Rahaq_0126)

MetaCyc: 72% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]

Predicted SEED Role

"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.5.4

Use Curated BLAST to search for 1.1.5.4

Protein Sequence (549 amino acids)

>EX31_RS05365 malate:quinone oxidoreductase (Rahnella sp. WP5)
MRKILAMILCLNVVAFSQSALAEDEKPVDVLLIGGGIMSATLGTYLQELEPDWSIDMVER
LDGVALESSNGWNNAGTGHSALAEMNYTPEKADGSIDISKAVEINESFQISRQFWTYQVE
NGVLHNPRSFINSTPHMSFVWGDDNINFLRKRFAALQQSTLFRGMEYSEDPAQIKQWIPL
VMEGRDPAQKIAATRIPIGTDVNFGEITRQLVASLQKKPNFSLSLGHEVRDIKRNPDNTW
NVTVADLKNDGKESVIKAKFVFIGAGGASLTLLQKSGIPEADNYGGFPVGGEFLVTENPD
VVKRHLAKVYGKASVGSPPMSVPHLDTRILDGKQVLLFGPFATFSSKFLKNGSLWDLFGS
VNFSNIMPMTHVGIDNFDLVKYLVSQVMLSDDDRFDALREYFPDAKKEDWRLWEAGQRVQ
IIKKDEKEGGVLRLGTEVVSSKDGSITALLGASPGASTAAPIMLYLMEKVFKDKVATPEW
QAKLKEIIPSYGTELNGNVAATEKELGDTSRVLMLDNPQPKAAEPAPAADNSSAPKKGEE
QKQVADVAL