Protein Info for EX31_RS05355 in Rahnella sp. WP5

Annotation: malate dehydrogenase (quinone)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06039: Mqo
  • amino acids 27 to 515 (489 residues), 778.6 bits, E=2.2e-238
• TIGR01320: malate dehydrogenase (acceptor)
  • amino acids 28 to 512 (485 residues), 690.3 bits, E=7e-212
• PF01266: DAO
  • amino acids 29 to 301 (273 residues), 41.3 bits, E=1.5e-14

Best Hits

Swiss-Prot: 71% identical to MQO_SERP5: Probable malate:quinone oxidoreductase (mqo) from Serratia proteamaculans (strain 568)

KEGG orthology group: None (inferred from 100% identity to rah:Rahaq_0133)

MetaCyc: 67% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]

Predicted SEED Role

"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.5.4

Use Curated BLAST to search for 1.1.5.4

Protein Sequence (550 amino acids)

>EX31_RS05355 malate dehydrogenase (quinone) (Rahnella sp. WP5)
MRKILGMLLNRDSLGASEPAAAEDEKSVDVLLVGGGIMSATLGTYLQELEPDWSVHMIER
MDGVAQESSNGWNNAGTGHSALAEMNYTPLMPDGSVDITKAVAINEAFQVSRQFMAYQVQ
KGVLNNPRSFINSVPHMSFVWGDENIDFLRKRFAALQESTLFRGMEYSEDPEQIRQWIPA
VMDGRDPKQKVAATRTTAGTDVNFGEVTRQLVEALQKKSNFTLANGQEVRDIQRNPDKSW
RVTITDLKHSHKHRVVNAKFVFIGAGGASLTLLQKSGIPEAREYGGFPVGGEFLVTENQD
VVKRHLAKVYGKAAVGAPPMSVPHLDTRIIDGKQVLLFGPFATFSSKFLKNGSLWDLFGS
VNSSNLLPMTHVGMGNFDLVKYLVGQVMLSDEDRHAALKEFFPEAEQKDWKRWVAGQRVQ
VIKKDGKHGGVLRLGTEVVSAKDGSIAALLGASPGASTAAPIMMKLLETVFKEKAASPEW
QAKFREIIPSYGTKLNGNIEATEKELLETSRILQLETPSMITTPVAEVVQLRPLDVQAKQ
EPKQPADIAL