Protein Info for EX31_RS05340 in Rahnella sp. WP5

Annotation: beta-galactosidase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1018 PF02837: Glyco_hydro_2_N" amino acids 42 to 204 (163 residues), 162.9 bits, see alignment E=1.7e-51 PF00703: Glyco_hydro_2" amino acids 209 to 318 (110 residues), 48.7 bits, see alignment E=2.9e-16 PF02836: Glyco_hydro_2_C" amino acids 321 to 601 (281 residues), 385.3 bits, see alignment E=4.6e-119 PF16353: LacZ_4" amino acids 611 to 696 (86 residues), 49.8 bits, see alignment E=8.8e-17 PF02929: Bgal_small_N" amino acids 726 to 994 (269 residues), 221.1 bits, see alignment E=5e-69

Best Hits

Swiss-Prot: 60% identical to BGA2_ECOLI: Evolved beta-galactosidase subunit alpha (ebgA) from Escherichia coli (strain K12)

KEGG orthology group: K12111, evolved beta-galactosidase subunit alpha [EC: 3.2.1.23] (inferred from 61% identity to ecc:c3833)

Predicted SEED Role

"Evolved beta-D-galactosidase, alpha subunit" in subsystem Lactose utilization

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1018 amino acids)

>EX31_RS05340 beta-galactosidase subunit alpha (Rahnella sp. WP5)
MNNWENIEKQSENRLPPRASFFSYENPQAALCFDRAASHGFQLLSGRWQFRFFEHPALVP
DEFYNQPMTEWGHITVPGMWQMEGHGQLQYTDEGFPFPIDVPYVPTNNPTGAYQQRFTLD
AAWAGKQTLIKFDGVETYFEVYVNGHYVGFSKGSRLCAEFDISASVKPGENLLSVRVLQW
ADSTYIEDQDMWWMAGIFRDVYLIGQDPVHLNDFTVVTTFDENYCDAVLNIDAVLGNLHH
PVSGYRLHAQLFDDGVCVAQLSADNLNIGDETRCRLDIPVTHPRQWNAEHPELYQLLLTL
YDDTGQMISVVPQRVGFRDIGVRDGLFYVNGRYLKLHGVNRHDHDHRKGRAVDMARVERD
IILMKQHNINSVRTAHYPNDPRFYELCDIYGLFVMAETDLESHGFANVGDLSRISDDPHW
EHAYVERIGRHVMAQKNHPSIIMWSLGNESGYGCNIRAMAKHCKALDPTRLVHYEEDRDA
EVTDVISTMYSRVQMMNAFGEYPHAKPRIICEYAHAMGNGPGGLAEYQAVLNRHDSLQGH
YIWEWCDHGLLETGENGQMRYTYGGDYGDYPNNYNFCMDGLIYPDQTPGPGLREYKQVLC
PVGVSLRGGALHIKNRYWFSSLEDITLSLTVQVAGRGLETREISLPHLQPGEGETLALPE
FPASGQEVFLNVGIRKNSPTVYSEAQHPLGQYQMLLQPARPSAMKPAPQKAATLLCDEQK
HQLIVSGEGFRLAFSRLDGELKSWQVDDEEITGRAPKLNFFKPVIDNHKQEFEGLWQPHH
LHIMQQHFRQMHWARQQDDVVIEVQSIIAPPVFDFGMRCTYRWQISPQGVVTLDLSGSPY
GNFDSIIPKIGLDFGVSRRFTQVEYYGRGPDENYQDSCQANLIGHYQQPVSRLFENYPMP
QDNGNRQDIRWLSLCDDAGKGIFIQPRQPLNFSLWPYSAQMLQQAQHIDELTPDGCLTLN
LDHKISGLGSNSWGSEVLDSYRVYFSAFRYGFTMVPFSQHDTNSQQLAEFTFHPREEN