Protein Info for EX31_RS05325 in Rahnella sp. WP5

Annotation: alpha-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 793 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF21152: YgjK_N" amino acids 42 to 295 (254 residues), 387.5 bits, see alignment E=5.7e-120 PF01204: Trehalase" amino acids 583 to 770 (188 residues), 30.7 bits, see alignment E=3.2e-11

Best Hits

Swiss-Prot: 76% identical to YGJK_ECOLI: Glucosidase YgjK (ygjK) from Escherichia coli (strain K12)

KEGG orthology group: K03931, putative isomerase (inferred from 76% identity to ecr:ECIAI1_3227)

MetaCyc: 76% identical to glycoside hydrolase (Escherichia coli K-12 substr. MG1655)
3.2.1.-

Predicted SEED Role

"Putative isomerase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (793 amino acids)

>EX31_RS05325 alpha-glucosidase (Rahnella sp. WP5)
MKKINPHRPLSLIICAALFISAHAQAENADTLRADSFKNVINRTGAPQYMLDYDFDEHQR
FNPLFDEGAWHGHLLPSGPQGMGGFPGPALLTEEYINFMADNFDRLTVYKAGQKVALSMT
AYSIPGALIQTLTAPGIRIDMTLRFVTSRTSLLETKITTDSPLELVWDGELLEKLRAQEG
KPPSDKTIVQEYPGYTRQILPVADGLRVIFGKVREASSLMTSGESEYQIHKSLPMQMTVT
GHQFTGKAKIAGSTTFYTTYSHLLTAAEVQKEQPKIQDILAHPQPYLDASQKRWEGYLNA
GLTNPKATKDQERVAVKAIETLTGNWRGAAGAMKFDSVTPSVTGRWFSGNQTWPWDTWKQ
AYAMAHFNPEVAKNNIRAVFAFQIQPGDPVRPWDAGFVPDLIAYNPGPERGGDGTNWNER
NTKPSLAAWSVMEIYKTTGDKQWLAEMYPKLVAYHDWWLHNRDHNHNGVPEYGATRDKAH
NTADGKMLFTVREGQKEQTESGLKNYDRVVKSGQYDSIEIPAQVAASWESGRDDAAAFGF
IDADQLTKYIKNGGKKQDWQVKFAENRSDDGTLLGYSLLQESVDEASYMYSDNKYMAEIA
DILGKKTAAADFRAKAVKLAGYINTCMFDSRSGFFYDIRIEDKPLANGCAGKPIVERGKG
PEGWSPLFNGAATQAHADAVVKVMKDVREFNTYVPLGTAALTNPAFGADIYWRGRVWVDQ
LYFGLKGMEQYGYREDAVKMAQAFFDHADGLVSDAPIRENYNPLNGQQQGAPNFSWSAAH
LYMLYNDFFGQGK