Protein Info for EX31_RS05025 in Rahnella sp. WP5

Annotation: 1,4-alpha-glucan branching enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 727 PF22019: GlgB_N" amino acids 7 to 99 (93 residues), 84.7 bits, see alignment E=7.8e-28 amino acids 109 to 206 (98 residues), 36.6 bits, see alignment E=8e-13 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 109 to 724 (616 residues), 991.3 bits, see alignment E=9.1e-303 PF02922: CBM_48" amino acids 124 to 207 (84 residues), 91.5 bits, see alignment E=6.9e-30 PF00128: Alpha-amylase" amino acids 273 to 342 (70 residues), 28.1 bits, see alignment E=2.6e-10 PF02806: Alpha-amylase_C" amino acids 628 to 725 (98 residues), 84.7 bits, see alignment E=9e-28

Best Hits

Swiss-Prot: 77% identical to GLGB_SERP5: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Serratia proteamaculans (strain 568)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 76% identity to eca:ECA4151)

MetaCyc: 70% identical to 1,4-alpha-glucan branching enzyme (Escherichia coli K-12 substr. MG1655)
1,4-alpha-glucan branching enzyme. [EC: 2.4.1.18]

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (727 amino acids)

>EX31_RS05025 1,4-alpha-glucan branching enzyme (Rahnella sp. WP5)
MSVLPDQHVINQIISGHYADPFSLLGMHSVAAGLEVRALLPDADQVWVIDVEKGKQVAEL
RRYDDRGFFVGLIPRRKNAFRYKLEVRWGENLHVIDDPYRFGTLLQELDIWLLAEGTHLR
PYEKLGAHLQTLDDVEGVSFAVWAPNATRVSVVGEFNFWDGRRHPMRQRRENGIWELFIP
AVREGQLYKFEIIDCRGHVSLRADPYAFEAQMRPDTASCVRKLPDVVPHSENRKKANALN
QPVSVYEVHLGSWRRHTDDNFWLSYGELAEQLVSYAKWMGFTHIELMPVNEHPFDGSWGY
QPLGMYAPTRRFGTPMEFKAFVDAAHNAGLNVILDWVPGHFPSDTYGLAQFDGTALYEYA
DPREGYHQDWNTLIYNYGRHEVRNYLAGNGFYWIERFGIDGLRVDAVASMIYRDYSRKAG
EWIPNYYGGRENLEAISFLKYTNQTIGRELGGAVTLAEESTDYPGVTMPPDANGLGFHYK
WNMGWMHDSLTYMQLDPVHRKYHHNLMTFGVLYAYSENFILPLSHDEVVHGKKSLLDKMP
GDVWQKFANLRAYYGFMWAHPGKKLLFMGNEFAQGREWNFDASLDWHLLDDPSGWHSGVQ
HLVRDLNQTYQANAPLYELDYNPRGFEWLVVDDHENSIFAFARRDENGGEVIVISNFTPV
PREHYRIGISQPGRYHEILNTDSHFYCGSNVGNGCDIYSENIGHHQRDHSISVTIPPLAT
VYLRREA