Protein Info for EX31_RS05000 in Rahnella sp. WP5
Annotation: glycerol-3-phosphate dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 79% identical to GLPD_ECOLI: Aerobic glycerol-3-phosphate dehydrogenase (glpD) from Escherichia coli (strain K12)
KEGG orthology group: K00111, glycerol-3-phosphate dehydrogenase [EC: 1.1.5.3] (inferred from 100% identity to rah:Rahaq_0207)MetaCyc: 79% identical to aerobic glycerol 3-phosphate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Glycerol-3-phosphate dehydrogenase. [EC: 1.1.5.3]
Predicted SEED Role
"Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Respiratory dehydrogenases 1 (EC 1.1.5.3)
MetaCyc Pathways
- glycerol and glycerophosphodiester degradation (4/4 steps found)
- glycerol degradation I (3/3 steps found)
- glycerol-3-phosphate shuttle (2/2 steps found)
- glycerol-3-phosphate to fumarate electron transfer (2/2 steps found)
- glycerophosphodiester degradation (2/2 steps found)
- nitrate reduction IX (dissimilatory) (2/2 steps found)
- glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer (1/2 steps found)
- glycerol-3-phosphate to cytochrome bo oxidase electron transfer (1/2 steps found)
- glycerol-3-phosphate to hydrogen peroxide electron transport (1/2 steps found)
- sn-glycerol 3-phosphate anaerobic respiration (1/3 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.1.5.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (503 amino acids)
>EX31_RS05000 glycerol-3-phosphate dehydrogenase (Rahnella sp. WP5) METKDLIVIGGGINGTGIAVDAAGRGLSVLLLEAQDLACATSSASSKLIHGGLRYLEHYE FRLVSEALAEREVLLKMAPHIAFPMRFRLPHQPHLRPAWMIRAGLFLYDHLGKRTSLPAS KGLKFGSESVLKPELTKGFEYSDCWVDDARLVVVNAQEVEERGGEVRTRTRVTRAWREDG MWMVEAEDIDTGKTFTWRAKGLVNATGPWVKHFFDDGLKLKSPYGIRLIKGSHIVVPRAH NQPQAYILQNEDHRIVFVIPWMDEFSIIGTTDVEYHGDPKDVKIDDKEIGYLLKVYNDHF KKQLTTEDIVWTYSGVRPLCDDESDSPQAVTRDYTLDVHDQDGKAPLLSVFGGKLTTYRK LAEHALEKLVKYYPKAGPAWTKTAVLPGGKLNGDRDTYSANLRSRFKWLPESLARRYART YGSQTELFLNDATSLESLGEDFGHGFYEAELRYLVEKEWVSQLDDAIWRRTKLGMWLDKT QQQRVAEWLETHKQQKKHLSLAS