Protein Info for EX31_RS04695 in Rahnella sp. WP5

Annotation: tryptophan--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00579: tRNA-synt_1b
  • amino acids 3 to 289 (287 residues), 286.2 bits, E=1.5e-89
• TIGR00233: tryptophan--tRNA ligase
  • amino acids 3 to 334 (332 residues), 383.6 bits, E=4.3e-119

Best Hits

Swiss-Prot: 84% identical to SYW_ECOL6: Tryptophan--tRNA ligase (trpS) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01867, tryptophanyl-tRNA synthetase [EC: 6.1.1.2] (inferred from 100% identity to rah:Rahaq_0274)

MetaCyc: 84% identical to tryptophan--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Tryptophan--tRNA ligase. [EC: 6.1.1.2]; 6.1.1.2 [EC: 6.1.1.2]

Predicted SEED Role

"Tryptophanyl-tRNA synthetase (EC 6.1.1.2)" (EC 6.1.1.2)

MetaCyc Pathways

• tRNA charging (21/21 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.2

Protein Sequence (338 amino acids)

>EX31_RS04695 tryptophan--tRNA ligase (Rahnella sp. WP5)
MSKPIVFSGAQPSGELTIGNYMGALRQWVKMQDDYECIYCIVDLHAITVRQDAEKLRKAT
LDTLALYLAVGIDPKKSTIFVQSHVPEHSQLSWALNCYTYFGELGRMTQFKDKSARYETS
NSESITAGLFDYPVLMAADILLYQTNQVPVGEDQKQHLELSRDIAARFNALYGDVFTVPE
PFIPKSGARVMSLQEPTKKMSKSDDNRKNVIGLLEDPKAVTKKIKSAMTDSEEPPVIRYD
IKNKPGVSNLLDILSGVTGKAIPELEAEFEGQMYGHLKGAVAEAVSGMLTEVQERFNRFR
SDEAYLQQVMKEGAEKARARASVTLKKAYEAIGFVSYE