Protein Info for EX31_RS04680 in Rahnella sp. WP5

Annotation: uroporphyrinogen-III C-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 460 TIGR01470: siroheme synthase, N-terminal domain" amino acids 4 to 206 (203 residues), 222.6 bits, see alignment E=4.2e-70 PF13241: NAD_binding_7" amino acids 6 to 115 (110 residues), 86.7 bits, see alignment E=2.9e-28 PF14824: Sirohm_synth_M" amino acids 119 to 145 (27 residues), 35.3 bits, see alignment (E = 1.2e-12) PF10414: CysG_dimeriser" amino acids 150 to 207 (58 residues), 54.8 bits, see alignment 1.3e-18 TIGR01469: uroporphyrinogen-III C-methyltransferase" amino acids 216 to 448 (233 residues), 299.9 bits, see alignment E=1.6e-93 PF00590: TP_methylase" amino acids 218 to 426 (209 residues), 176.9 bits, see alignment E=1.1e-55

Best Hits

Swiss-Prot: 73% identical to CYSG2_YERP3: Siroheme synthase 2 (cysG2) from Yersinia pseudotuberculosis serotype O:1b (strain IP 31758)

KEGG orthology group: K02302, uroporphyrin-III C-methyltransferase / precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 2.1.1.107 4.99.1.4] (inferred from 100% identity to rah:Rahaq_0275)

MetaCyc: 72% identical to uroporphyrinogen-III C-methyltransferase subunit (Salmonella enterica enterica serovar Typhimurium)
Sirohydrochlorin ferrochelatase. [EC: 4.99.1.4]; Uroporphyrinogen-III C-methyltransferase. [EC: 4.99.1.4, 2.1.1.107]; 2.1.1.107 [EC: 4.99.1.4, 2.1.1.107]; Precorrin-2 dehydrogenase. [EC: 4.99.1.4, 2.1.1.107, 1.3.1.76]

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis or Coenzyme B12 biosynthesis or Dissimilatory nitrite reductase (EC 1.3.1.76, EC 2.1.1.107, EC 4.99.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.76, 2.1.1.107, 4.99.1.4

Use Curated BLAST to search for 1.3.1.76 or 2.1.1.107 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (460 amino acids)

>EX31_RS04680 uroporphyrinogen-III C-methyltransferase (Rahnella sp. WP5)
MDYLPLFCQLKNRACLLVGAGDVAERKARLLLEAGAKLTVNALTFTPQFQIWADEGKVRL
LRGEFSPLLVDEKWLVIAATDSDLVNQEVSEAADHRRVFCNVVDAPESASVIMPSIIDRS
PLMIAVSSGGNAPVLARLLRERLESILPQHLGKLAQVAGGLRARVKTHFPDASERRRWWE
KLFNHHRLAQSLANNDNVQSHEYLEELFREPVDKHGEVILVGAGPGDAGLLTLKGLQQMQ
LADVVVYDRLVSDSVLNLARRDAERIFVGKRAGHHCVPQERINEILLEQAQMGKRVVRLK
GGDPFIFGRGGEELEALKAAGIAFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLITGHA
KQDGVLDWACMAQTQQTLVFYMGLTQAGEIQRRLLENGLAAETPVALVENGTSQQQRVVT
GKLTELEILAKKVQSPSLIIVGSVVSLRQKLNWFASDTCL