Protein Info for EX31_RS04670 in Rahnella sp. WP5

Annotation: nitrite reductase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 PF07992: Pyr_redox_2" amino acids 5 to 292 (288 residues), 180.7 bits, see alignment E=1.6e-56 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 6 to 801 (796 residues), 1267.1 bits, see alignment E=0 PF13738: Pyr_redox_3" amino acids 82 to 280 (199 residues), 34.6 bits, see alignment E=4.6e-12 PF00070: Pyr_redox" amino acids 148 to 225 (78 residues), 60.2 bits, see alignment E=7.9e-20 PF18267: Rubredoxin_C" amino acids 321 to 389 (69 residues), 38.6 bits, see alignment E=2.9e-13 PF04324: Fer2_BFD" amino acids 424 to 471 (48 residues), 55.8 bits, see alignment 1.7e-18 PF03460: NIR_SIR_ferr" amino acids 560 to 621 (62 residues), 46.3 bits, see alignment 9.9e-16 PF01077: NIR_SIR" amino acids 633 to 768 (136 residues), 67.5 bits, see alignment E=3.3e-22

Best Hits

Swiss-Prot: 82% identical to NIRB_ECOLI: Nitrite reductase (NADH) large subunit (nirB) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 83% identity to eae:EAE_05120)

MetaCyc: 82% identical to nitrite reductase (NADH) large subunit (Escherichia coli K-12 substr. MG1655)
RXN-13854 [EC: 1.7.1.15]

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.15 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (850 amino acids)

>EX31_RS04670 nitrite reductase large subunit (Rahnella sp. WP5)
MSKVRIAIIGNGMVGHRFIEDLLDKAEPGQFDITVFCEEPRVAYDRVHLSSYFSHHTAEE
LSLVREGFYEKHNVNVLIGERAITLNRSEKVIHSSSGRTVYYDKLIIATGSYPWVPPIAG
SDSQDCFVYRTIEDLHAIESCARRSKRGAVVGGGLLGLEAAGALKNLGVETHVVEFAPGL
MAEQLDTQGGSQLRQKIESMGVRVHTAKNTQQIVQGGTSARKTMEFADGTFLEVDFIVFS
TGIRPQDKLARQCDLEIAPRGGIVINDQCQTSDPDVYAIGECASWNQRTFGLVAPGYKMA
QVTADHLLGRDNAFTGADMSAKLKLLGVDVAGIGDAHARTPGARSYVYLDETKALYKRLV
VSEDNKTLLGAVLVGDTSDYGNLLQLVLNSIPLPENPDALILPAHAGSGGAVIGTDALPE
SAQICSCFDVTKGDIIKAVQGGCHTVAALKSATKAGTGCGGCIPLVTQVLNAELSKQGIE
VNHHLCEHFAYSRQELYHLIRVEGIRSFDDLLNKYGKGYGCEVCKPTVGSLLASCWNDYV
LKPQHTPLQDTNDVFLANMQKDGTYSVIPRSPGGEITPQGLQAIGEIAAEYNLYTKITGS
QRIGMFGAQKDDLPAIWQKLINAGFETGQAYAKALRMAKTCVGSTWCRYGVGDSLGFGIS
LENRYKGIRTPHKMKFGVSGCTRECSEAQGKDVGIIATENGWNLYVCGNGGMKPRHGDLL
AADLDNDTLLRYLDRFMMFYIRTADKLQRTSVWMDNLEGGIDYLRKVIIDNKLGLNDQLE
AELTRLRDSAVCEWQETLNDPAAQTRFSHFINSSQRDPNVQFVGERQQHRPARPDERIPV
RQIADGEEVL