Protein Info for EX31_RS04665 in Rahnella sp. WP5

Annotation: cytosine deaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 PF07969: Amidohydro_3" amino acids 46 to 403 (358 residues), 229 bits, see alignment E=1.8e-71 PF01979: Amidohydro_1" amino acids 54 to 381 (328 residues), 60.8 bits, see alignment E=1.4e-20

Best Hits

Swiss-Prot: 85% identical to CODA_ECOLI: Cytosine deaminase (codA) from Escherichia coli (strain K12)

KEGG orthology group: K01485, cytosine deaminase [EC: 3.5.4.1] (inferred from 99% identity to rah:Rahaq_0278)

MetaCyc: 85% identical to cytosine/isoguanine deaminase (Escherichia coli K-12 substr. MG1655)
Cytosine deaminase. [EC: 3.5.4.1]; RXN0-6708 [EC: 3.5.4.1]

Predicted SEED Role

"Cytosine deaminase (EC 3.5.4.1)" (EC 3.5.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.1

Use Curated BLAST to search for 3.5.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (427 amino acids)

>EX31_RS04665 cytosine deaminase (Rahnella sp. WP5)
MTKTLLRTVKNVRLPDREGLWQIDIEGGVIRQIVPQWDNADYGEHILDAEGGLAIPPFIE
PHIHLDTTQTAGQPAWNQSGTLFEGIERWAERKALLSHEDVKQRAWQTLKWQIANGIQYV
RTHVDVSDPTLTALKAMLEVKAEVAPWVTLQIVAFPQEGIMSYPNGEALLEEALKLGADV
VGAIPHFEFTREYGVESLHKTFELAKKYDKLIDVHCDEIDDEQSRFVETVAALALRENMG
ARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFIANPLVNIHLQGRFDTYPKRRGITRVK
EMLEAGINVCFGHDDVFDPWYPLGTANMLQVLHMGLHVCQLMGYQQINDGLKLITTYSAR
TMNLQGYGLESGYAASLVILPAQNGFDAVRRQTPVQYSLREGVLIAQTQLAKTLIHLDED
HPVDFNS