Protein Info for EX31_RS03945 in Rahnella sp. WP5

Annotation: bifunctional aspartate kinase/homoserine dehydrogenase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00696: AA_kinase
  • amino acids 2 to 284 (283 residues), 150.6 bits, E=1.6e-47
• TIGR00657: aspartate kinase
  • amino acids 3 to 460 (458 residues), 483.6 bits, E=3.4e-149
• PF13840: ACT_7
  • amino acids 314 to 376 (63 residues), 36 bits, 1.3e-12
  • amino acids 394 to 452 (59 residues), 37.1 bits, 5.7e-13
• PF03447: NAD_binding_3
  • amino acids 472 to 605 (134 residues), 92 bits, E=1.1e-29
• PF00742: Homoserine_dh
  • amino acids 614 to 810 (197 residues), 165.9 bits, E=2e-52

Best Hits

Swiss-Prot: 87% identical to AK1H_SERMA: Bifunctional aspartokinase/homoserine dehydrogenase 1 (thrA) from Serratia marcescens

KEGG orthology group: K12524, bifunctional aspartokinase / homoserine dehydrogenase 1 [EC: 1.1.1.3 2.7.2.4] (inferred from 85% identity to eca:ECA3891)

MetaCyc: 83% identical to fused aspartate kinase/homoserine dehydrogenase 1 (Escherichia coli K-12 substr. MG1655)
Aspartate kinase. [EC: 2.7.2.4]; Homoserine dehydrogenase. [EC: 2.7.2.4, 1.1.1.3]

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis or Methionine Biosynthesis (EC 1.1.1.3, EC 2.7.2.4)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (8/8 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of L-threonine biosynthesis (6/6 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• L-lysine biosynthesis III (6/7 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• L-homoserine biosynthesis (3/3 steps found)
• L-lysine biosynthesis II (7/9 steps found)
• dipicolinate biosynthesis (3/4 steps found)
• norspermidine biosynthesis (4/6 steps found)
• ectoine biosynthesis (3/5 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• L-methionine biosynthesis IV (2/4 steps found)
• spermidine biosynthesis II (2/4 steps found)
• superpathway of polyamine biosynthesis III (4/8 steps found)
• 3-dehydroquinate biosynthesis II (archaea) (3/7 steps found)
• cremeomycin biosynthesis (2/7 steps found)
• grixazone biosynthesis (2/8 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones
• Glycine, serine and threonine metabolism
• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.3, 2.7.2.4

Use Curated BLAST to search for 1.1.1.3 or 2.7.2.4

Protein Sequence (819 amino acids)

>EX31_RS03945 bifunctional aspartate kinase/homoserine dehydrogenase I (Rahnella sp. WP5)
MRVLKFGGTSVANAERFLRVADIMESNARQGQVATVLSAPAKITNYLVAMIERTVAGQDI
QTIMSDAENIFGQLITGLAAAQPGFDLPKVKAFVDHEFAQLKQLMHGIALLGQCPDSVNA
SIICRGEKLSIAIMEAVFLAKGFGVTVINPVEKLLAQGHYLESTVDINESTRRIAASAIP
ADHIVLMAGFTAGNEQGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRIV
PDARLLKSMSYQEAMELSYFGAKVLHPRTILPIAQFQIPCLIKNTTNPQAPGTLIGSESL
GDNTPVKGITNLNNMAMINVSGPGMKGMIGMAARVFAVMSRSGISVVLITQSSSEYSISF
CVPQSELERARKALEDEFYLELKDGLLEPLDVMEKLAVISVVGDGMRTLRGISAKFFSAL
ARANINIVAIAQGSSERSISVVVNNDDATTGVRVSHQMLFNTDQVIEVFLIGVGGVGGAL
IEQIRRQQAWLKTKHIDLRVCGIANSRALLTNVHGIALETWRDELGAAKEPLNLGRLIRL
VKEYHLLNPVIVDCTSSQEVADQYADFLADGFHVVTPNKKANTSSMNYYHQLRKAADGSR
RKFLYDTNVGAGLPVIENLQNLLNAGDELMRFSGILSGSLSFIFGKLDEGMSLSAATLQA
KEMGYTEPDPRDDLSGMDVARKLLILAREAGYKLELTDIDVESVLPPSFNSEGTVNEFVA
RLPELDAEFARRVADATAAGKVLRYVGAIEDGQCKVKIDAVDGNDPLYKVKNGENALAFY
SRYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV