Protein Info for EX31_RS03870 in Rahnella sp. WP5

Annotation: isoleucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 938 TIGR00392: isoleucine--tRNA ligase" amino acids 15 to 842 (828 residues), 992.5 bits, see alignment E=9.5e-303 PF00133: tRNA-synt_1" amino acids 28 to 640 (613 residues), 516.8 bits, see alignment E=1.2e-158 PF09334: tRNA-synt_1g" amino acids 58 to 201 (144 residues), 38.3 bits, see alignment E=2.2e-13 PF01406: tRNA-synt_1e" amino acids 576 to 648 (73 residues), 27.5 bits, see alignment 6.2e-10 PF08264: Anticodon_1" amino acids 685 to 840 (156 residues), 104 bits, see alignment E=2.1e-33 PF06827: zf-FPG_IleRS" amino acids 898 to 926 (29 residues), 43.6 bits, see alignment (E = 6e-15)

Best Hits

Swiss-Prot: 85% identical to SYI_CITK8: Isoleucine--tRNA ligase (ileS) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 85% identity to cko:CKO_03366)

MetaCyc: 84% identical to isoleucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Isoleucine--tRNA ligase. [EC: 6.1.1.5]; 6.1.1.5 [EC: 6.1.1.5]; 3.1.1.- [EC: 6.1.1.5]; RXN-23924 [EC: 6.1.1.5]

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (938 amino acids)

>EX31_RS03870 isoleucine--tRNA ligase (Rahnella sp. WP5)
MSDYKNTLNLPETGFPMRGDLAKREPDMLKNWYDQDLYGIIRAAKKGKKTFILHDGPPYA
NGSIHIGHSVNKILKDMIVKSKGLAGFDAPYVPGWDCHGLPIELKVEQLIGKPGEKVTAA
EFREACRKYAAEQVEGQKKDFIRLGVLGDWDHPYLTMDFKTEANIIRALGKIISNGHLHK
GAKPVHWCTDCGSSLAEAEVEYYDKASPSIDVAFNATDAAAVAAKFGVSAFNGPISLVIW
TTTPWTMPANRAISLNPEFSYQLVQVEGQCLILATDLVESVMKRAGIAEWTVLGECKGAD
LELLRFKHPFLGFDVPAILGDHVTLDAGTGAVHTAPGHGPDDFVIGQKYGLEVANPVGPN
GCYLPGTYPTLDGKFVFKANDLIVELLREKGALLHVEKITHSYPCCWRHKTPIIFRATPQ
WFISMDQKGLRQQSLEEIKGVQWIPDWGQARIENMVANRPDWCISRQRTWGVPMSLFVHK
DTEQLHPRSLELMEEVAKRVEVDGIQAWWDLNPEDILGADAAEYVKVPDTLDVWFDSGST
HSSVVDVRPEFNGHSPDLYLEGSDQHRGWFMSSLMISTAMKGKAPYKQVLTHGFTVDGQG
RKMSKSIGNTIAPQDVMNKLGGDILRLWVASTDYTGEIAVSDEILKRAADSYRRIRNTAR
FLLANLNGFDPALHSVAPEDMVVLDRWAVGRAKAAQDEIIAAYEAYDFHGVVQRLMQFCS
IEMGSFYLDIIKDRQYTAKSDSVARRSCQTALYHISEALVRWMAPIMSFTADEIWAELPG
SREKFVFTEEWYDGLFGLAGNESMNDAFWDELLKVRGEVNKVIEQARADKRLGGSLEAAV
TLFADDALATDLRSLGNELRFVLLTSGAKVAALSEADDATVPSELLKGLKIGLAKAEGDK
CPRCWHYTTDVGQNAEHSDICGRCVTNIAGDGEERKFA