Protein Info for EX31_RS03605 in Rahnella sp. WP5

Annotation: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 TIGR01133: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase" amino acids 1 to 347 (347 residues), 369.6 bits, see alignment E=7.8e-115 PF03033: Glyco_transf_28" amino acids 9 to 145 (137 residues), 161.1 bits, see alignment E=3.4e-51 PF13439: Glyco_transf_4" amino acids 25 to 157 (133 residues), 31.2 bits, see alignment E=4.3e-11 PF04101: Glyco_tran_28_C" amino acids 185 to 340 (156 residues), 130.7 bits, see alignment E=1.1e-41

Best Hits

Swiss-Prot: 82% identical to MURG_PECAS: UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K02563, UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [EC: 2.4.1.227] (inferred from 100% identity to rah:Rahaq_3731)

MetaCyc: 81% identical to N-acetylglucosaminyl transferase (Escherichia coli K-12 substr. MG1655)
acetylglucosaminyltransferase. [EC: 2.4.1.227]

Predicted SEED Role

"UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227)" in subsystem Peptidoglycan Biosynthesis (EC 2.4.1.227)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.227

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (355 amino acids)

>EX31_RS03605 undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (Rahnella sp. WP5)
MSGNTRRLMVMAGGTGGHVFPGLAVAHHLIAQGWEVRWLGTADRMEADLVPKHGIEIDFI
KIAGLRGKGLKAQLTAPVRIYHAWRQAKAIMKRFQPDVVLGMGGYVSGPGGLAAWSLGIP
VVLHEQNGIAGMTNKGLSHIAKKVLQAFPGAFPNADVVGNPVRTDVLALELPATRLAGRE
GPIRVLVIGGSQGARVLNQSMPEVAALMGDKITLWHQVGKGALASVNQAYEKAGQTQHKV
TEFIDDMAEAYAWADVVVCRSGALTVSEIAAAGLPAIFVPFQHKDRQQYWNALPLEKAGA
AKIIEQKDFSAAAVADLMAGWDRTHLMEMAMAARAAAIPDATERVAAEVVAASKK