Protein Info for EX31_RS03560 in Rahnella sp. WP5

Annotation: preprotein translocase subunit SecA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 905 PF07517: SecA_DEAD" amino acids 7 to 401 (395 residues), 431.4 bits, see alignment E=4e-133 TIGR00963: preprotein translocase, SecA subunit" amino acids 28 to 818 (791 residues), 1145 bits, see alignment E=0 PF01043: SecA_PP_bind" amino acids 232 to 358 (127 residues), 137 bits, see alignment E=9.9e-44 PF21090: P-loop_SecA" amino acids 417 to 615 (199 residues), 306 bits, see alignment E=3.1e-95 PF07516: SecA_SW" amino acids 618 to 831 (214 residues), 246.1 bits, see alignment E=8.3e-77 PF02810: SEC-C" amino acids 884 to 902 (19 residues), 40.8 bits, see alignment (E = 4e-14)

Best Hits

Swiss-Prot: 71% identical to SECA_ALIFM: Protein translocase subunit SecA (secA) from Aliivibrio fischeri (strain MJ11)

KEGG orthology group: K03070, preprotein translocase subunit SecA (inferred from 73% identity to aha:AHA_3877)

Predicted SEED Role

"Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (905 amino acids)

>EX31_RS03560 preprotein translocase subunit SecA (Rahnella sp. WP5)
MLIKLLTKVFGSRNDRTLRRMRKVVDLINRMEPEIQKLTDEQLQAKTNEFRERLAKGEVL
ENLIPEAFAVVRESSKRVFGMRHFDVQLLGGMVLNERCIAEMRTGEGKTLTATLPAYLNA
LSGKGVHVVTVNDYLAQRDAENNRPLFEFLGLSIGINLPGMPSPAKRAAYAADITYGTNN
EYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDEARTPLIISGPAEDSSEMYTRVD
KLIPKLIRQEKEDSDSFQGEGHFSVDEKSRQVHLTERGLVLIEELLVEAGIMEEGESLYS
PTNIMLMHHVTAALRAHVLFTRDVDYIVKDGEVIIVDEHTGRTMQGRRWSDGLHQAVEAK
EGVDIQNENQTLASITFQNYFRLYEKLAGMTGTADTEAFEFSSIYKLDTIVVPTNRPMVR
KDMADLVYMTEMEKIGAIIEDIRRCTAKGQPVLVGTISIEKSEVVSNELTKAGIAHSVLN
AKFHAKEADIVAQAGQPSAVTIATNMAGRGTDIVLGGSWQVEVENLGEEATDEKIAEIKA
AWQIRHDAVLASGGLHIIGTERHESRRIDNQLRGRAGRQGDAGSSRFYLSMEDALMRIFA
SDRVSSMMRKLGMKPGEAIEHPWVTKAIANAQRKVESRNFDIRKQLLEYDDVASDQRRAI
YSQRNELLDVSDVSETIASIREDVFKATIDSYIPPQSLEEMWDVQGLEERLKKDFELEMP
IAEWLDKEPELHEETLRERIMEIAKESYARKEEVVGAEMMRNFEKGVMLQTLDSLWKEHL
AAMDYLRQGIHLRGYAQKDPKQEYKRESFAMFAAMLESLKYEVVSVLSKVQVRMPEEVEA
MEIQRREEAERLARQQQLSHQDQQEIEIAEAARLASSGDRKVGRNDLCPCGSGKKYKQCH
GRLQK