Protein Info for EX31_RS03475 in Rahnella sp. WP5

Annotation: pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00364: Biotin_lipoyl
  • amino acids 4 to 73 (70 residues), 79.3 bits, E=5.6e-26
  • amino acids 108 to 178 (71 residues), 76.8 bits, E=3.4e-25
  • amino acids 210 to 280 (71 residues), 77.3 bits, E=2.3e-25
• TIGR01348: dihydrolipoyllysine-residue acetyltransferase
  • amino acids 187 to 632 (446 residues), 755.4 bits, E=2.2e-231
• PF02817: E3_binding
  • amino acids 328 to 363 (36 residues), 57.1 bits, 5.7e-19
• PF00198: 2-oxoacid_dh
  • amino acids 401 to 631 (231 residues), 270.8 bits, E=3.2e-84

Best Hits

Swiss-Prot: 87% identical to ODP2_ECOLI: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (aceF) from Escherichia coli (strain K12)

KEGG orthology group: K00627, pyruvate dehydrogenase E2 component (dihydrolipoamide acetyltransferase) [EC: 2.3.1.12] (inferred from 99% identity to rah:Rahaq_3704)

MetaCyc: 87% identical to pyruvate dehydrogenase E2 subunit (Escherichia coli K-12 substr. MG1655)
Dihydrolipoyllysine-residue acetyltransferase. [EC: 1.2.1.104, 2.3.1.12]; 1.2.1.104 [EC: 1.2.1.104, 2.3.1.12]

Predicted SEED Role

"Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 2.3.1.12)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• pyruvate fermentation to acetate V (2/3 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (10/17 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.104 or 2.3.1.12

Protein Sequence (632 amino acids)

>EX31_RS03475 pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase (Rahnella sp. WP5)
MAIEINVPDIGADAVEVTEIMVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVA
VGDTVETGKLIMIFDAADGAADAAPAKQEAKAEEKPAAAAAPASSESKEVNVPDIGGDEV
EVTEIMVKVGDTVEAEQSLITVEGDKASMEVPAPFAGKVKEIKIAAGDKVSTGSLIMVFE
VAGSGAAAPAAAAETQSDAAPAAAAQSGSKEVNVPDIGGDEVEVTEIMVKVGDKISAEQS
LITVEGDKASMEVPAPFAGTVKEIKIAAGDKVSTGSLIMVFEVEGAAPAAPAAKKEEAAA
PAKQEQKAAAPAVKAESKGEFAENDAYVHATPVIRRLAREFGVNLAKVKGTGRKGRILRE
DVQTYVKDAVKRAEAAPAAATGGGLPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSR
NWVMIPHVTHFDKTDITELEAFRKQQNDEAAKRKLDVKFTPVVFIMKAVAAALEQMPRFN
SSLSEDGQKLTLKKYINIGVAVDTPNGLVVPVFKDVNKKGIAELSRELMAISKKARDGKL
TAGEMQGGCFTISSLGGIGTTHFAPIVNAPEVAILGVSKSAIEPVWNGKEFTPRLMMPMS
LSFDHRVIDGADGARFITIINNVLSDIRRLVM