Protein Info for EX31_RS03230 in Rahnella sp. WP5

Annotation: bifunctional glycosyl transferase/transpeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 876 transmembrane" amino acids 81 to 102 (22 residues), see Phobius details PF14812: PBP1_TM" amino acids 1 to 95 (95 residues), 85 bits, see alignment E=7.1e-28 TIGR02071: penicillin-binding protein 1B" amino acids 84 to 821 (738 residues), 1164.9 bits, see alignment E=0 PF14814: UB2H" amino acids 130 to 214 (85 residues), 83.8 bits, see alignment E=1.5e-27 PF00912: Transgly" amino acids 228 to 398 (171 residues), 193.3 bits, see alignment E=5e-61 PF00905: Transpeptidase" amino acids 492 to 731 (240 residues), 91.5 bits, see alignment E=1.2e-29

Best Hits

Swiss-Prot: 68% identical to PBPB_ECOLI: Penicillin-binding protein 1B (mrcB) from Escherichia coli (strain K12)

KEGG orthology group: K05365, penicillin-binding protein 1B [EC: 2.4.1.129 3.4.-.-] (inferred from 70% identity to ebi:EbC_07920)

MetaCyc: 68% identical to peptidoglycan glycosyltransferase / peptidoglycan DD-transpeptidase MrcB (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]; 3.4.16.4 [EC: 3.4.16.4]; Peptidoglycan glycosyltransferase. [EC: 3.4.16.4, 2.4.1.129]

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.-.-, 3.4.16.4

Use Curated BLAST to search for 2.4.1.129 or 3.4.-.- or 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (876 amino acids)

>EX31_RS03230 bifunctional glycosyl transferase/transpeptidase (Rahnella sp. WP5)
MSGDDREPIGRKGKPAGRKPARARPVRRSRRDDDYEEYEEEIDDEYEDDDDYEEERESRM
SRKAPPRKANKGGKKSARRRLFWLLVKLFVVFLILLGIYAVYLNKQIHDRIDGGKVWQLP
AAVYGRQVNLEPDMQISKAEMVGLLEGTQYREVTRITRPGEFTVHANYIDLLRRPFDFPE
GKEGQVNARLMFDGNSLSEIKNLDNQRDFGLFRLDPRLITMLQASNANGEQRLFVPRSGF
PDLLVDTLLATEDRHFYEHDGINFYSIGRAVVANLTAGHAVQGGSTLTQQLVKNLFLTNE
RSLSRKAREAVMAILLDADYSKDRILELYLNEVYLGQSGSDQIRGFPLASLYYFGRPVNE
LSLDQQALLVGMVKGASLYNPWRNPQMALQRRNLVLRLLVDQKVIDQELYDMLSARPLGV
QPKGGVISPQPAFMQMVRQELQAKLGDKVQDMSGVKIFTTLDPISQDAAEKAVEEGIPLL
RKKSGLNDIETAMVVVDRYTGEVRAMVGGAETQFAGFNRAMQARRQVGSLAKPATYLTAL
SQPDKYRLNTILADQPLTLKLANGQTWSPKNDDHQFRGQVLLVDALARSMNVPTVNLGMA
VGLDNITKTLIDLGIPKESLNQTPSMLLGAIALTPMEVAQEYQTIASGGNKAPLSAVRSV
IAEDGTVLYQSFPQAVRVIPSQAAYLTLYAMQQVVQEGTSRALANKFPKLHLAAKTGTSN
ELRDSWFAGIDGNQVAITWVGRDNNGPSKLWGSTGALVLYGRYLENGTPQPLDLQMPEGI
STMNIDGSGNFVCGNSGGLLGGSGAVRSIPVWTDSPDAMCQASVSAVQQQQQQIQQQQQQ
QQQQQSGQQQQPQSQDEKKDSNGVAGWIKDMFGSGN