Protein Info for EX31_RS03200 in Rahnella sp. WP5

Annotation: H(+)/Cl(-) exchange transporter ClcA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 transmembrane" amino acids 35 to 55 (21 residues), see Phobius details amino acids 76 to 99 (24 residues), see Phobius details amino acids 129 to 147 (19 residues), see Phobius details amino acids 179 to 203 (25 residues), see Phobius details amino acids 215 to 234 (20 residues), see Phobius details amino acids 254 to 275 (22 residues), see Phobius details amino acids 292 to 310 (19 residues), see Phobius details amino acids 330 to 351 (22 residues), see Phobius details amino acids 359 to 381 (23 residues), see Phobius details amino acids 391 to 415 (25 residues), see Phobius details amino acids 423 to 442 (20 residues), see Phobius details PF00654: Voltage_CLC" amino acids 93 to 438 (346 residues), 324.8 bits, see alignment E=3.5e-101

Best Hits

Swiss-Prot: 75% identical to CLCA_CITK8: H(+)/Cl(-) exchange transporter ClcA (clcA) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K03281, chloride channel protein, CIC family (inferred from 100% identity to rah:Rahaq_3652)

MetaCyc: 67% identical to chloride:H+ antiporter ClcA (Escherichia coli K-12 substr. MG1655)
RXN0-2501

Predicted SEED Role

"H(+)/Cl(-) exchange transporter ClcA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (487 amino acids)

>EX31_RS03200 H(+)/Cl(-) exchange transporter ClcA (Rahnella sp. WP5)
MNESDTEILADAPVRGPRKRSEILRLLLRRDKTPVTILLVAAVVGTVAGLLGVAFEKAVN
WVMAVRLAGLGVFNDYWILLWPLTFVFSAALAMVGYYLVRCYAPEAGGSGIPEIEGALEE
LRPVRWWRVIPVKFFGGMGTLGAGMVLGREGPTVQMGANVGKMMVDIFRLRSAEARHSLL
ATGAAAGLSAAFNAPLAGILFILEEMRLQFRYSLISIKAVFIGVIMSSIVFRIFNGDGAV
IEVGRLASAPLNTLWLYLVLGMVFGVVGVSFNALIFRTQDMFARVHGGNLRKILIIGGML
GGFCGLLGLVHQEVTGGGFALIPLAAAGKYSLMMLLVIFVVRVVTTLLCFASGAPGGIFA
PMLALGTLLGTAFGTAALAWFPQYGIQPATFAIAGMGALFAASVRAPLTGIVLVLEMTDN
YQLILPMIITCLGATLLAQFLGGKPLYSAILTRTLQRQERENAEKAQGEIAAEGINKQDS
AAKNAHT