Protein Info for EX31_RS02925 in Rahnella sp. WP5

Annotation: polysaccharide biosynthesis tyrosine autokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 726 transmembrane" amino acids 32 to 51 (20 residues), see Phobius details amino acids 424 to 448 (25 residues), see Phobius details PF02706: Wzz" amino acids 15 to 106 (92 residues), 70 bits, see alignment E=3.4e-23 PF13807: GNVR" amino acids 367 to 446 (80 residues), 109 bits, see alignment E=1.8e-35 TIGR01007: capsular exopolysaccharide family" amino acids 509 to 710 (202 residues), 158.1 bits, see alignment E=1.1e-50 PF13614: AAA_31" amino acids 540 to 652 (113 residues), 42.6 bits, see alignment E=1.4e-14

Best Hits

Swiss-Prot: 53% identical to WZC_ECO57: Tyrosine-protein kinase wzc (wzc) from Escherichia coli O157:H7

KEGG orthology group: K00903, protein-tyrosine kinase [EC: 2.7.10.-] (inferred from 64% identity to rah:Rahaq_3596)

MetaCyc: 53% identical to protein-tyrosine kinase Wzc (Escherichia coli K-12 substr. MG1655)
Receptor protein-tyrosine kinase. [EC: 2.7.10.1, 2.7.10.2, 2.7.10.3, 2.7.12.1]

Predicted SEED Role

"Tyrosine-protein kinase Wzc (EC 2.7.10.2)" in subsystem Colanic acid biosynthesis (EC 2.7.10.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.10.2

Use Curated BLAST to search for 2.7.10.- or 2.7.10.1 or 2.7.10.2 or 2.7.10.3 or 2.7.12.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (726 amino acids)

>EX31_RS02925 polysaccharide biosynthesis tyrosine autokinase (Rahnella sp. WP5)
MTSAFTKKPTVPDSDEIELGLIIGELLDHKKLIISITGIFTIIAILYVLFSTPVFQADAL
VQVEQKQGNVLLENLSQVLPEAQPQSEPEIALIQSRMILGKTVDDLNLQAQIQQNYFPLF
GQGWARLTGAKTGSLKVTKLYIPQIGSDKQEVTLTVIDKNNFKVEGKSFSFSGQTGKVLE
KPEVTILVDEIVAEPGTSFTITYQSKLSAIEKLQKNLLVVDQGKDSGMLTLTLTGEDRAQ
ISAILNRITQNYLAQNIARQAAQDAKSLEFLQSQLPKVRSDLDDAEDKLNAYRKKQDSID
LSLEAKSVLDQIVNVDNQLNQLTFSEAEISQLYTKENPKYKALLEKRKVLEKNRLSLNQQ
VSAMPSTQQEVVRLSRNVDSGRAVYMQLLNRQQELEIAKSSAIGNVRIIDHAVTDPIPVK
PKKALIVAISFILGGFISVGVILFKAFFHRGIESPEQLENLGISVYASIPSSDWLLKNSV
INTGKKVKGKNKDNTSFLAVDNPADISIEALRSLRTSLHFAMLEATNNILMISGPSPRAG
KSFVSANLAAVMAQADKKILLIDGDMRKGIAHKMLKVGYEMGLSDLISSSISFESGVKNI
KSANFDFISRGQVAPNPAELLMHARFQELLNWASAKYDLVIIDTPPILAVTDAAIIGRHA
GTTLLVARFEQNTIKEVEVSARRFEQSGVTIKGCILNGIIKKASSYYGYGYNHYGYDYSD
NKNPRA