Protein Info for EX31_RS02735 in Rahnella sp. WP5

Annotation: A/G-specific adenine glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 358 TIGR01084: A/G-specific adenine glycosylase" amino acids 13 to 278 (266 residues), 417.5 bits, see alignment E=1.4e-129 PF00730: HhH-GPD" amino acids 43 to 174 (132 residues), 94.9 bits, see alignment E=7.3e-31 PF00633: HHH" amino acids 107 to 134 (28 residues), 33.7 bits, see alignment (E = 4.3e-12) PF10576: EndIII_4Fe-2S" amino acids 199 to 215 (17 residues), 20 bits, see alignment (E = 1.3e-07) PF14815: NUDIX_4" amino acids 241 to 348 (108 residues), 78.2 bits, see alignment E=8.4e-26

Best Hits

Swiss-Prot: 74% identical to MUTY_ECOLI: Adenine DNA glycosylase (mutY) from Escherichia coli (strain K12)

KEGG orthology group: K03575, A/G-specific adenine glycosylase [EC: 3.2.2.-] (inferred from 100% identity to rah:Rahaq_3556)

MetaCyc: 74% identical to adenine DNA glycosylase (Escherichia coli K-12 substr. MG1655)
RXN0-2661 [EC: 3.2.2.31]

Predicted SEED Role

"A/G-specific adenine glycosylase (EC 3.2.2.-)" in subsystem DNA repair, bacterial (EC 3.2.2.-)

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.-

Use Curated BLAST to search for 3.2.2.- or 3.2.2.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (358 amino acids)

>EX31_RS02735 A/G-specific adenine glycosylase (Rahnella sp. WP5)
MMETPGFMEAKSFSPLVLDWYQRYGRKTLPWQIAKNPYKVWLSEVMLQQTQVATVIPYFE
RFMERFPTVSDLAAAPLDEVLHLWTGLGYYARARNLHKAAQTIVSQHSGVFPTTFDEILA
LPGIGRSTAGAVLSLALNQHYPILDGNVKRVLARCYAVDGWPGEKKTENKLWAISEDVTP
AEGVAQFNQAMMDLGAMVCTRSKPKCELCPVKSGCEAYAHHSWAKYPGKKPKTTLPEKTA
FMLMIQQEDKVWLEQRPSVGLWGGLFCFPQYSTEDELTLGLQKYGVTQNALQQQTAFRHT
FSHFHLDIVPMWLNLRSPAGCMDEGAGLWYNLAQPQSVGLAAPVERLLMQLAKQTAGK