Protein Info for EX31_RS01830 in Rahnella sp. WP5

Annotation: 5-histidylcysteine sulfoxide synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR04344: 5-histidylcysteine sulfoxide synthase
  • amino acids 17 to 459 (443 residues), 692.7 bits, E=2.2e-212
• PF12867: DinB_2
  • amino acids 36 to 171 (136 residues), 31.6 bits, E=7.6e-11
• PF03781: FGE-sulfatase
  • amino acids 207 to 459 (253 residues), 74.6 bits, E=3.6e-24
• TIGR04345: putative 4-mercaptohistidine N1-methyltranferase
  • amino acids 472 to 712 (241 residues), 330.1 bits, E=8e-103
• PF13489: Methyltransf_23
  • amino acids 500 to 676 (177 residues), 42 bits, E=3e-14
• PF13847: Methyltransf_31
  • amino acids 513 to 669 (157 residues), 40.3 bits, E=9.9e-14
• PF13649: Methyltransf_25
  • amino acids 515 to 555 (41 residues), 27.1 bits, 1.9e-09
• PF08241: Methyltransf_11
  • amino acids 515 to 571 (57 residues), 23.3 bits, 2.9e-08
  • amino acids 568 to 638 (71 residues), 25.6 bits, E=5.5e-09

Best Hits

KEGG orthology group: None (inferred from 99% identity to rah:Rahaq_4559)

Predicted SEED Role

"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13

Use Curated BLAST to search for 1.4.1.13

Protein Sequence (713 amino acids)

>EX31_RS01830 5-histidylcysteine sulfoxide synthase (Rahnella sp. WP5)
MLSTQNKAIGLPEPTQTILLSTGTAEEKRQEIRDYFIQTWELYESLFDCLADERAYFNKA
ISLRHPLIFYFGHTATFYINKLMAAGLIPQRVDSHIEAMLAIGVDEMSWDDLDDQNYNWP
SVVNLRQYRDKVREEVLGIIDTMALSLPIGWDSPAWVILMGIEHERIHLETSSVLIRQLP
VEWVKPQSHWPVCGIARHDRNSVPDNTMLAVKGDIVTLGKQDDTYGWDNEYGTSQQKIQA
FSASKMLVSNAEFYTFIQADGYHDAQWWDEEGLGWRDFARAEMPTFWVGSPREPERLKLR
LMTEEVPMPWDWPAEVNQLEAAAFCRWKAAQTGESVQLPTEAEWMILRNQVQGDQPDWET
APGNINLAWWASSCPVDHFEQGDFSDVVGNVWQWASTPISGFDGFRVHPLYDDFSTPTFD
GKHSMIKGGSWISTGNEALKSSRYAFRRHFFQHAGFRYVISSHQEVVKVNPYETDGLVAQ
YLDFQYGPRHFDVTNYAVQLVDIAVENTVNRGRALDIGCATGRASFELARHFDSVVGMDY
SARFIDVALALARGEACRYVIPEEGELVDYCQASLADVNISAEQTVRVNFVQGDACNLKP
QAELYDLVLAANLLDRLREPARFLTDITPMIRSGGLLMLSSPYTWLEEFTPKEHWLGGFR
ENGEAVTTHQALKRLLANEFEEVRKPTDVPFVIRETARKFQHTVAEVTLWRKR