Protein Info for EX31_RS01815 in Rahnella sp. WP5

Annotation: (S)-acetoin forming diacetyl reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 6 to 199 (194 residues), 209.6 bits, E=5e-66
• TIGR02415: acetoin reductases
  • amino acids 6 to 259 (254 residues), 398.3 bits, E=6.7e-124
• PF08659: KR
  • amino acids 8 to 167 (160 residues), 46.3 bits, E=7e-16
• PF13561: adh_short_C2
  • amino acids 15 to 256 (242 residues), 194.6 bits, E=3e-61

Best Hits

Swiss-Prot: 84% identical to BUDC_KLEPN: Diacetyl reductase [(S)-acetoin forming] (budC) from Klebsiella pneumoniae

KEGG orthology group: K03366, (R,R)-butanediol dehydrogenase / diacetyl reductase [EC: 1.1.1.303 1.1.1.4] (inferred from 100% identity to rah:Rahaq_4555)

MetaCyc: 84% identical to acetoin reductase subunit (Klebsiella pneumoniae)
RXN-11032 [EC: 1.1.1.304]; 1.1.1.- [EC: 1.1.1.304]

Predicted SEED Role

"2,3-butanediol dehydrogenase, S-alcohol forming, (R)-acetoin-specific (EC 1.1.1.4) / Acetoin (diacetyl) reductase (EC 1.1.1.5)" in subsystem Acetoin, butanediol metabolism (EC 1.1.1.4, EC 1.1.1.5)

MetaCyc Pathways

• superpathway of (R,R)-butanediol biosynthesis (5/5 steps found)
• pyruvate fermentation to (R)-acetoin I (3/3 steps found)
• pyruvate fermentation to (S)-acetoin (3/3 steps found)
• (R,R)-butanediol biosynthesis (1/1 steps found)
• (R,R)-butanediol degradation (1/1 steps found)
• meso-butanediol biosynthesis I (1/1 steps found)
• superpathway of 2,3-butanediol biosynthesis (4/6 steps found)

KEGG Metabolic Maps

• Butanoate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.303 or 1.1.1.304 or 1.1.1.4 or 1.1.1.5

Protein Sequence (259 amino acids)

>EX31_RS01815 (S)-acetoin forming diacetyl reductase (Rahnella sp. WP5)
MSKNGKVALVTGGGQGIGRAIALRLAKDGFAVAVVDFNGETAKKVAEEINHAGGKAIAQL
ADVSDREQVFAAVEAATRQLGGFDVIVNNAGIAPTTLIEDITPEIVDKVYNINVKGVIWG
IQAAVKAFKSQGHGGKIINASSQAGHVGNPELAIYSSSKFAVRGLTQTAARDLASLGITV
NAYCPGIVKTPMWEEIDRQVSAAAGKPAGYGTQEFAKNITLKRLSEPEDVAACVSYLAGP
DSDYMTGQALLIDGGMVFN