Protein Info for EX31_RS01490 in Rahnella sp. WP5

Annotation: efflux RND transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 388 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 33 to 369 (337 residues), 272.6 bits, see alignment E=1.9e-85 PF16576: HlyD_D23" amino acids 58 to 288 (231 residues), 58.8 bits, see alignment E=7.5e-20 PF13533: Biotin_lipoyl_2" amino acids 58 to 105 (48 residues), 59.9 bits, see alignment 2.5e-20

Best Hits

Swiss-Prot: 32% identical to MEXA_PSEAE: Multidrug resistance protein MexA (mexA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 100% identity to rah:Rahaq_4492)

Predicted SEED Role

"Membrane fusion protein of RND family multidrug efflux pump" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (388 amino acids)

>EX31_RS01490 efflux RND transporter periplasmic adaptor subunit (Rahnella sp. WP5)
MTLRLSGVALMVALLAGCDNSVAQNAAPPAPQVSAADVLIKPISQWDAFNGRVEAVQSVQ
LRPRVSGYIDKVNYQEGQEVKKGQVLFTIDDRTYRAAQEQAQAELVRARNQASLARSESA
RTEALIGSLAVSKEAAEQRRSAASQAQSDVLAAQAQLDMAQLNLDFTRVISPIDGRASRA
MITTGNLVTAGDSASVLTTVVSLDTVYVYFDVDEGTFLRYQAQAREGKFNDSPQSRLPVH
VGLVGENGYPHQGVVDFTDNQLNSGTGTIRMRALLDNRDRTFTPGLFARVQMPGSAQFNA
LLVDDKAILTDQDRKFVYVVDKDGKAQRRDVEIGRIYGGLRIVQKGLEAGDRVIIDGVQK
VFMPGMPVNAKTVSMAANTVTPAAPAVQ