Protein Info for EX31_RS01460 in Rahnella sp. WP5

Annotation: glycine betaine/L-proline transporter ProP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 transmembrane" amino acids 39 to 57 (19 residues), see Phobius details amino acids 64 to 87 (24 residues), see Phobius details amino acids 99 to 119 (21 residues), see Phobius details amino acids 125 to 153 (29 residues), see Phobius details amino acids 166 to 188 (23 residues), see Phobius details amino acids 199 to 218 (20 residues), see Phobius details amino acids 261 to 279 (19 residues), see Phobius details amino acids 299 to 316 (18 residues), see Phobius details amino acids 328 to 347 (20 residues), see Phobius details amino acids 353 to 377 (25 residues), see Phobius details amino acids 389 to 412 (24 residues), see Phobius details amino acids 418 to 439 (22 residues), see Phobius details PF00083: Sugar_tr" amino acids 29 to 450 (422 residues), 245 bits, see alignment E=3.1e-76 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 34 to 433 (400 residues), 476.2 bits, see alignment E=4.4e-147 PF07690: MFS_1" amino acids 65 to 311 (247 residues), 90.5 bits, see alignment E=2.1e-29 amino acids 276 to 451 (176 residues), 62 bits, see alignment E=1e-20 PF13347: MFS_2" amino acids 78 to 406 (329 residues), 32.7 bits, see alignment E=6.2e-12 PF08946: Osmo_CC" amino acids 455 to 500 (46 residues), 73.8 bits, see alignment 2.1e-24

Best Hits

Swiss-Prot: 80% identical to OUSA_DICD3: Glycine betaine/proline/ectoine/pipecolic acid transporter OusA (ousA) from Dickeya dadantii (strain 3937)

KEGG orthology group: K03762, MFS transporter, MHS family, proline/betaine transporter (inferred from 100% identity to rah:Rahaq_4485)

MetaCyc: 79% identical to osmolyte:H+ symporter ProP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-29; TRANS-RXN-29A

Predicted SEED Role

"L-Proline/Glycine betaine transporter ProP" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (500 amino acids)

>EX31_RS01460 glycine betaine/L-proline transporter ProP (Rahnella sp. WP5)
MRLRKKRVKPMQINDITIIDDTKLKKAITAAALGNAMEWFDFGVYGFVAFALGQVFFPGA
DPGIQMIAALATFSVPFLVRPLGGLFFGALGDKFGRQKVLSVTIIIMSVSTFCIGLIPGY
NSIGIWAPILLLLAKLAQGFSVGGEYTGAAIFVAEYSPDRKRGFLGSWLDFGSIAGFILG
AGVVVLISSIVGEANFLEWGWRIPFFIAAPLGLIGIYLRHALEETPTFQQHVDKIDSDAR
KDIAEPPRVSFREILTKQWRPLIICVGMVIATNVTYYMLLTYMPSYLSHSLHYSEDHGVL
IIIAIMVGMLFVQPFMGMLSDRFGRKPFVIIGSIGLFILAIPCFILINSDVIGLIFLGLL
ILAVLLNCFIGVMASTLPAMFPTHIRYSALAIAFNISVLIAGLTPTIAAWLVETTNNLYM
PAYYLMVVAMIGLVTGIFMKETANRPLKGATPAASDRAEAREILQEHHDNIEQKIEDIDA
QIVELEAKRKNLIAQHPDIN