Protein Info for EX31_RS01395 in Rahnella sp. WP5

Annotation: DHA2 family efflux MFS transporter permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 481 transmembrane" amino acids 20 to 47 (28 residues), see Phobius details amino acids 50 to 59 (10 residues), see Phobius details amino acids 65 to 83 (19 residues), see Phobius details amino acids 91 to 110 (20 residues), see Phobius details amino acids 116 to 137 (22 residues), see Phobius details amino acids 149 to 167 (19 residues), see Phobius details amino acids 178 to 199 (22 residues), see Phobius details amino acids 214 to 232 (19 residues), see Phobius details amino acids 238 to 257 (20 residues), see Phobius details amino acids 278 to 305 (28 residues), see Phobius details amino acids 311 to 328 (18 residues), see Phobius details amino acids 340 to 363 (24 residues), see Phobius details amino acids 369 to 394 (26 residues), see Phobius details amino acids 406 to 426 (21 residues), see Phobius details amino acids 446 to 465 (20 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 27 to 422 (396 residues), 233.2 bits, see alignment E=2.9e-73 PF07690: MFS_1" amino acids 30 to 256 (227 residues), 126.6 bits, see alignment E=5.7e-41 amino acids 286 to 466 (181 residues), 52.3 bits, see alignment E=2.1e-18

Best Hits

Swiss-Prot: 38% identical to HSRA_ECOLI: Probable transport protein HsrA (hsrA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to rah:Rahaq_4472)

Predicted SEED Role

"Permeases of the major facilitator superfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (481 amino acids)

>EX31_RS01395 DHA2 family efflux MFS transporter permease subunit (Rahnella sp. WP5)
MSHSKPDASEHPQGDSAVNTGISVIALLVAAAFFMEFIDGTVIATALPQMAISFGVSAVD
LNAGMSAYMLTLAVLIPASGWAAERFGARNVFTFALGVFTLASLFCAMASSVSEFIILRI
IQGIGGALMVPVGRLTVLKTTPKPQLIKAIATLTWPALVAPILGPPLGGFITHYASWHWI
FYINVPLGIIAMMLAWRLFPQEPSAEPKHFDKPGFVLTGLAMLSLVTGLELISQDHILWT
AAILLLVTGVVLSVLAIRHLYRDPEPMVHLGAIVIPTFRISMGGGSLFRVTISAVPFLLP
LMLQVGFGMDPFHAGLLVLAVFAGNLAMKPATTPLIRRFGFRPVLVVNGLLSVFSLLFCA
FLTPDTPDWLVMVLLFLGGLSRSMQFTGISTLAFSDVPAKQMADANTLFSTVLQLSVGLG
ITVGALGTRLGEQLVDALDWASVPGMSFKISFVVIAVLTLIGWLDMLRLKPDAGSSVSGK
K