Protein Info for EX31_RS00855 in Rahnella sp. WP5

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01891: amidohydrolase
  • amino acids 14 to 369 (356 residues), 335.4 bits, E=2.3e-104
• PF01546: Peptidase_M20
  • amino acids 69 to 380 (312 residues), 154.5 bits, E=3.7e-49
• PF07687: M20_dimer
  • amino acids 184 to 276 (93 residues), 35.1 bits, E=1.1e-12

Best Hits

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 99% identity to rah:Rahaq_4898)

Predicted SEED Role

"N-acetyl-L,L-diaminopimelate deacetylase (EC 3.5.1.47)" in subsystem Lysine Biosynthesis DAP Pathway (EC 3.5.1.47)

MetaCyc Pathways

• L-lysine biosynthesis II (7/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis
• Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32, 3.5.1.47

Use Curated BLAST to search for 3.5.1.32 or 3.5.1.47

Protein Sequence (392 amino acids)

>EX31_RS00855 amidohydrolase (Rahnella sp. WP5)
MRSIPEEIIQQAIIWRREIHTRPEIGLQEFHTSAKISSLLRQYDLEVHTGIAGTGVMGVL
RNGEGPSIALRADIDALPIQENNAVAWCSSSPGMMHACGHDGHTAILLGAARYLSQTRAF
NGTVYFIFQPAEENAGGGRLMVEEGIFTRFPVSAVYALHNWPGLPVGEVAVSTGPMMASQ
DNFFITLTGVGCHAAMPEKGADPVLAGAHLITALQTIMTRRLSPLDSAVISVTQLQAGEA
INVVPQTMQLSGTLRCLSQTARARCQILLAEYVEQLVGPFGVSGSIRWEYGYPVTVNHAQ
QAKILADAARHVQGITQVHTQLSPSMAAEDFAYFLEACPGAYLWLGADGPTPGAALHNPH
YDFNDQLIAPGIGLWVSLVERSLGPVSVTSED