Protein Info for EX31_RS00730 in Rahnella sp. WP5

Annotation: aspartate aminotransferase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 490 PF00282: Pyridoxal_deC" amino acids 67 to 417 (351 residues), 214.5 bits, see alignment E=3.1e-67 PF01212: Beta_elim_lyase" amino acids 182 to 370 (189 residues), 34.9 bits, see alignment E=1.7e-12 PF05889: SepSecS" amino acids 192 to 292 (101 residues), 21.2 bits, see alignment E=1.6e-08

Best Hits

Swiss-Prot: 57% identical to DDC_ACIBA: L-2,4-diaminobutyrate decarboxylase (ddc) from Acinetobacter baumannii

KEGG orthology group: K13745, L-2,4-diaminobutyrate decarboxylase [EC: 4.1.1.86] (inferred from 100% identity to rah:Rahaq_4873)

Predicted SEED Role

"L-2,4-diaminobutyrate decarboxylase (EC 4.1.1.86)" (EC 4.1.1.86)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.86

Use Curated BLAST to search for 4.1.1.86

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (490 amino acids)

>EX31_RS00730 aspartate aminotransferase family protein (Rahnella sp. WP5)
MSELNPILAGSAQSTEAYQQAIAQSSEAVVQWLKQPEMYQGKTVAELRERIRLDFNPNGL
GNQAAIERAIEYFLKDSLSVHHPQCVAHLHCPSLVVSQAAEVLINATNQSMDSWDQSPSA
TIIEMKLVEWLRTQVGYQAGDAGVFTSGGTQSNLMGLMLARDAFFARQGHSIQQDGLVGN
LRKIKVLCSENAHFSVQKNMALLGLGYQCVTLVKTDEFARMDLNDLREKVAQAQASGDQI
LAIVATAGTTDAGAIDPLRAIAELAAEHQIWVHVDAAWGGALLLSEKYRNYLDGIELVDS
ITLDFHKQFFQTISCGAFLLKEERHYELMRYQAAYLNSEFDEAAGVPNLVSKSLQTTRRF
DALKLWMGLEALGQKQYAAIIDHGVTLAQEVAQYVTSEAALELVMKPQLASVLFRYRPEQ
LAGSSDAAIALLNQKIGDALLESGRANVGVTEHNGVTCLKLTLLNPTVTLEDVKVLLALV
EKTAQQLLNA