Protein Info for EX31_RS00275 in Rahnella sp. WP5

Annotation: glucose/quinate/shikimate family membrane-bound PQQ-dependent dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 799 transmembrane" amino acids 10 to 33 (24 residues), see Phobius details amino acids 39 to 57 (19 residues), see Phobius details amino acids 64 to 80 (17 residues), see Phobius details amino acids 86 to 109 (24 residues), see Phobius details amino acids 118 to 139 (22 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 14 to 794 (781 residues), 1165.1 bits, see alignment E=0 PF13360: PQQ_2" amino acids 197 to 381 (185 residues), 24.8 bits, see alignment E=2.5e-09 amino acids 676 to 777 (102 residues), 21.2 bits, see alignment E=3e-08 PF01011: PQQ" amino acids 226 to 259 (34 residues), 33.9 bits, see alignment (E = 2.7e-12) amino acids 293 to 324 (32 residues), 20.6 bits, see alignment (E = 4.2e-08) amino acids 360 to 387 (28 residues), 21.1 bits, see alignment (E = 2.9e-08) amino acids 447 to 472 (26 residues), 23.8 bits, see alignment (E = 4.2e-09) amino acids 673 to 703 (31 residues), 22.2 bits, see alignment (E = 1.4e-08) amino acids 726 to 760 (35 residues), 26.3 bits, see alignment (E = 7e-10)

Best Hits

Swiss-Prot: 71% identical to DHG_ECOLI: Quinoprotein glucose dehydrogenase (gcd) from Escherichia coli (strain K12)

KEGG orthology group: K00117, quinoprotein glucose dehydrogenase [EC: 1.1.5.2] (inferred from 80% identity to ebi:EbC_12100)

MetaCyc: 71% identical to quinoprotein glucose dehydrogenase (Escherichia coli K-12 substr. MG1655)
Quinoprotein glucose dehydrogenase. [EC: 1.1.5.2]

Predicted SEED Role

"Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2)" in subsystem D-gluconate and ketogluconates metabolism or Entner-Doudoroff Pathway or Respiratory dehydrogenases 1 (EC 1.1.5.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (799 amino acids)

>EX31_RS00275 glucose/quinate/shikimate family membrane-bound PQQ-dependent dehydrogenase (Rahnella sp. WP5)
METKASLSRIVVIITALFAALSGIYLLVGGIWLAKLGGSLYYIIAGVISLVTAWLLYRRR
SSALLLYAIFLFGTTVWAVWEVGTDFWALTPRLDVTFFLGLWILLPVVYNQMLAKNAFAR
GALAVSLLFTVIVLAYSVFNDPQEINGTLKAADAAPAATDSGVPASDWPAYGRTQGGTRY
SPLNQINDKNVSKLDVAWTFRTGDLKTPNDPGEITDEVTPIKIGDMLYLCTPHQKLFALD
AATGKEKWKFDPQLNSNPTFQHVTCRGVSYHETTPAAQGDAANGAAPAVCSRRIILPVND
GRLFALDAETGARCPAFGNNGELNLQGNMPYATPGHYEPTSPPIITKSVIIVAGAVTDNY
SNREPSGVIRGFDVETGKLLWAFDPGAAEPNKIPEDGQHFTPNSPNSWAPAAYDDKLDLV
YLPIGVATPDIWGGNRTPEMERYASGLLALNATTGKLAWFYQTVHHDLWDMDVPAQPTLA
DITDKSGNKVPAIYVPTKTGNIFVLDRRDGKLIVDAPEKPVPQGAAKGDHVSPTQPFSEL
TFRPEAKLTGKDMWGATIYDQLMCRVIFHKLRYEGTFTPPSEQGTLVFPGNLGMFEWGGI
SVDTDRQVAIANPIALPFVSKLIPRGPGNPMEPDENDKGGSGTETGIQPQYGVPFGVTLN
PFLSPLGFPCKQPSWGYISGVDLKTNDIVWKKRIGTVRDSSPLPLPFKMGMPMLGAPIST
AGNVFFIAATADNYLRAFDMSNGDKLWEARLPAGGQATPMTYSVNGKQYVVIAAGGHGSF
GTKLGDYIIAYALPDADAK