Protein Info for ECOLIN_RS27000 in Escherichia coli Nissle 1917

Annotation: S-methyl-5-thioribose-1-phosphate isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00512: S-methyl-5-thioribose-1-phosphate isomerase
  • amino acids 17 to 348 (332 residues), 410.1 bits, E=6.2e-127
• TIGR00524: eIF-2B alpha/beta/delta-related uncharacterized proteins
  • amino acids 41 to 347 (307 residues), 292.9 bits, E=2.6e-91
• PF01008: IF-2B
  • amino acids 52 to 347 (296 residues), 209.5 bits, E=3e-66

Best Hits

Swiss-Prot: 100% identical to MTNA_ECOLU: Methylthioribose-1-phosphate isomerase (mtnA) from Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)

KEGG orthology group: K08963, methylthioribose-1-phosphate isomerase [EC: 5.3.1.23] (inferred from 100% identity to ecz:ECS88_4902)

MetaCyc: 100% identical to methylthioribose-1-phosphate isomerase (Escherichia coli ATCC 25922)
S-methyl-5-thioribose-1-phosphate isomerase. [EC: 5.3.1.23]; 5.3.1.23 [EC: 5.3.1.23]

Predicted SEED Role

"Methylthioribose-1-phosphate isomerase (EC 5.3.1.23)" in subsystem Methionine Salvage (EC 5.3.1.23)

MetaCyc Pathways

• 5'-deoxyadenosine degradation II (4/4 steps found)
• 5'-deoxyadenosine degradation I (2/3 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (2/5 steps found)
• L-methionine salvage cycle I (bacteria and plants) (7/12 steps found)
• L-methionine salvage cycle III (6/11 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (1/5 steps found)
• L-methionine salvage cycle II (plants) (5/11 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation I (2/7 steps found)

KEGG Metabolic Maps

• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.3.1.23

Protein Sequence (371 amino acids)

>ECOLIN_RS27000 S-methyl-5-thioribose-1-phosphate isomerase (Escherichia coli Nissle 1917)
MNIKGKHYRTVWVSGDGKAVEIIDQTKLPFKFEVVALTSAEMAATAIQEMWVRGAPLIGV
VAAYGIALGMNHDASDMGLQRYYDLLIKTRPTAINLKWALDRMIDTLKDLCVSERKDVAW
ALAAEIAEEDVALCEQIGLHGTEVIREIAQKKPAGSVVNILTHCNAGWLATVDWGTALSP
IYKAHENGIPVHVWVDETRPRNQGGLTAFELGSHGIPHTLIADNAGGHLMQHGDVDLCIV
GTDRTTARGDVCNKIGTYLKALAAHDNHVPFYVALPSPTIDWTIEDGKSIPIEQRDGKEQ
SHVYGINPQGELSWVNTAPEGTRCGNYAFDVTPARYITGFITERGVCAASKSALADMFAD
LKSKALQGEQH