Protein Info for ECOLIN_RS26870 in Escherichia coli Nissle 1917

Annotation: isocitrate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00463: ICL
  • amino acids 8 to 255 (248 residues), 206.3 bits, E=8e-65
  • amino acids 257 to 434 (178 residues), 168.3 bits, E=2.5e-53
• TIGR01346: isocitrate lyase
  • amino acids 8 to 255 (248 residues), 413 bits, E=8.2e-128
  • amino acids 256 to 434 (179 residues), 313.2 bits, E=1.4e-97
• PF13714: PEP_mutase
  • amino acids 81 to 288 (208 residues), 54.2 bits, E=1.6e-18

Best Hits

Swiss-Prot: 100% identical to ACEA_ECOLI: Isocitrate lyase (aceA) from Escherichia coli (strain K12)

KEGG orthology group: K01637, isocitrate lyase [EC: 4.1.3.1] (inferred from 100% identity to eco:b4015)

MetaCyc: 100% identical to isocitrate lyase (Escherichia coli K-12 substr. MG1655)
Isocitrate lyase. [EC: 4.1.3.1]

Predicted SEED Role

"Isocitrate lyase (EC 4.1.3.1)" in subsystem Serine-glyoxylate cycle (EC 4.1.3.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.1

Use Curated BLAST to search for 4.1.3.1

Protein Sequence (434 amino acids)

>ECOLIN_RS26870 isocitrate lyase (Escherichia coli Nissle 1917)
MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECTLAQLGAAKMWRLLHGE
SKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLAASMYPDQSLYPANSVPAV
VERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEAGFGGVLNAFELMKAMIEAGA
AAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARLAADVTGVPTLLVARTDADAADL
ITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRGLAYAPYADLVWCETSTPDLELARR
FAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIASFQQQLSDMGYKFQFITLAGIHSMWF
NMFDLANAYAQGEGMKHYVEKVQQPEFAAAKDGYTFVSHQQEVGTGYFDKVTTIIQGGTS
SVTALTGSTEESQF