Protein Info for ECOLIN_RS24305 in Escherichia coli Nissle 1917

Annotation: isovaleryl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF18158: AidB_N
  • amino acids 10 to 167 (158 residues), 187.9 bits, E=2.5e-59
• PF02770: Acyl-CoA_dh_M
  • amino acids 181 to 275 (95 residues), 63.7 bits, E=2.9e-21
• PF00441: Acyl-CoA_dh_1
  • amino acids 285 to 438 (154 residues), 104.9 bits, E=9.5e-34
• PF08028: Acyl-CoA_dh_2
  • amino acids 309 to 420 (112 residues), 32.9 bits, E=1.4e-11

Best Hits

Swiss-Prot: 99% identical to AIDB_ECOLI: Putative acyl-CoA dehydrogenase AidB (aidB) from Escherichia coli (strain K12)

KEGG orthology group: K09456, putative acyl-CoA dehydrogenase (inferred from 99% identity to eco:b4187)

MetaCyc: 99% identical to isovaleryl-CoA dehydrogenase and DNA-binding transcriptional repressor (Escherichia coli K-12 substr. MG1655)
RXN0-2301 [EC: 1.3.8.4]

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

• oleate β-oxidation (30/35 steps found)
• fatty acid salvage (5/6 steps found)
• adipate degradation (4/5 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• L-leucine degradation I (2/6 steps found)

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7

Use Curated BLAST to search for 1.3.8.4 or 1.3.8.7

Protein Sequence (541 amino acids)

>ECOLIN_RS24305 isovaleryl-CoA dehydrogenase (Escherichia coli Nissle 1917)
MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGASWDSDFLASIGQQLGTAESLELGR
LANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHNLAWEEDARSGAFVARAAR
FMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPLLSDRYDSHLLPGGQKRGLLI
GMGMTEKQGGSDVMSNTTRAERLDDGSYRLVGHKWFFSVPQSDAHLVLAQTAGGLSCFFV
PRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDAIGWLLGQEGEGIRLILKMGGMTRF
DCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQPLMRHVLSRMALQLEGQTALLFRLAR
AWDRRADAKEALWARLFTPAAKFVICKRGIPFVAEAMEVLGGIGYCEESELPRLYREMPV
NSIWEGSGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQQQLRKPAEE
LGREITHQLFLLGCGAQMLKYASPPMAQAWCKVMLDTRGGVRLSEQIQNDLLLRATGGVC
L