Protein Info for ECOLIN_RS23415 in Escherichia coli Nissle 1917

Annotation: AAA family ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13521: AAA_28
  • amino acids 173 to 324 (152 residues), 112 bits, E=1.8e-36

Best Hits

KEGG orthology group: None (inferred from 100% identity to ecc:c5030)

Predicted SEED Role

"Nicotinamide-nucleotide adenylyltransferase, NadR family (EC 2.7.7.1) / Ribosylnicotinamide kinase (EC 2.7.1.22)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation or PnuC-like transporters (EC 2.7.1.22, EC 2.7.7.1)

MetaCyc Pathways

• NAD salvage pathway IV (from nicotinamide riboside) (2/2 steps found)
• NAD biosynthesis from nicotinamide (1/2 steps found)
• superpathway of NAD biosynthesis in eukaryotes (7/14 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.22, 2.7.7.1

Use Curated BLAST to search for 2.7.1.22 or 2.7.7.1

Protein Sequence (334 amino acids)

>ECOLIN_RS23415 AAA family ATPase (Escherichia coli Nissle 1917)
MKPFATGLVVGKFAPLHCGHEKLINTALAQCEELFIISYSVPEMPDCEPEKRLTWLQVRF
PQATILVLTPELVARYNLPAIPHNDADADIHRHYVATLCLQILRCRPHAVFTAEDYGDGF
ANVLARRFAQPVEHVRMARPVGDEAPSGTLIRSDVHRYRYMLANDVYYSFVRRICLLGGE
STGKSTLSKALADGLDTVYVAEFGRDYWEEKNGILTADDLLHIACEQVRREQQAEANHYL
ICDTSPLTTLFYALDQYGHAPQELHQLAEREYSLVVLCGAEFPFVQDGTRQGEVFRARQQ
VWYEQELSRRNIPYLSVSGPLQERVEQILRQLPD