Protein Info for ECOLIN_RS22935 in Escherichia coli Nissle 1917

Annotation: bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 transmembrane" amino acids 123 to 138 (16 residues), see Phobius details amino acids 144 to 162 (19 residues), see Phobius details TIGR00122: biotin operon repressor" amino acids 6 to 76 (71 residues), 101.6 bits, see alignment E=1.7e-33 PF08279: HTH_11" amino acids 9 to 58 (50 residues), 48.5 bits, see alignment 9.6e-17 TIGR00121: biotin--[acetyl-CoA-carboxylase] ligase" amino acids 81 to 317 (237 residues), 289.5 bits, see alignment E=1.9e-90 PF03099: BPL_LplA_LipB" amino acids 84 to 208 (125 residues), 110 bits, see alignment E=1.3e-35 PF02237: BPL_C" amino acids 271 to 317 (47 residues), 50 bits, see alignment 3.4e-17

Best Hits

Swiss-Prot: 98% identical to BIRA_ECOLI: Bifunctional ligase/repressor BirA (birA) from Escherichia coli (strain K12)

KEGG orthology group: K03524, BirA family transcriptional regulator, biotin operon repressor / biotin-[acetyl-CoA-carboxylase] ligase [EC: 6.3.4.15] (inferred from 98% identity to eok:G2583_4788)

MetaCyc: 98% identical to DNA-binding transcriptional repressor/biotin-[acetyl-CoA-carboxylase] ligase BirA (Escherichia coli K-12 substr. MG1655)
Biotin--[acetyl-CoA-carboxylase] ligase. [EC: 6.3.4.15]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (321 amino acids)

>ECOLIN_RS22935 bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA (Escherichia coli Nissle 1917)
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSL
PEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELKSGDACVAEYQHAGRGRRG
RKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQ
DRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAA
MLIRELRAALELFEQEGLTPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEK