Protein Info for ECOLIN_RS22865 in Escherichia coli Nissle 1917

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 transmembrane" amino acids 20 to 37 (18 residues), see Phobius details amino acids 53 to 74 (22 residues), see Phobius details amino acids 85 to 108 (24 residues), see Phobius details amino acids 111 to 128 (18 residues), see Phobius details amino acids 149 to 169 (21 residues), see Phobius details amino acids 177 to 197 (21 residues), see Phobius details amino acids 239 to 259 (21 residues), see Phobius details amino acids 276 to 300 (25 residues), see Phobius details amino acids 312 to 331 (20 residues), see Phobius details amino acids 337 to 359 (23 residues), see Phobius details amino acids 371 to 394 (24 residues), see Phobius details amino acids 400 to 421 (22 residues), see Phobius details PF07690: MFS_1" amino acids 28 to 364 (337 residues), 180.6 bits, see alignment E=2.1e-57

Best Hits

Swiss-Prot: 56% identical to LGOT_ECOLI: Probable L-galactonate transporter (lgoT) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eck:EC55989_4444)

MetaCyc: 56% identical to galactonate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-227

Predicted SEED Role

"D-galactonate transporter" in subsystem D-galactonate catabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (427 amino acids)

>ECOLIN_RS22865 MFS transporter (Escherichia coli Nissle 1917)
MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSVAGEAIRGELGLSATEF
GVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQALTGMVNSFSHFILMRIG
LGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPPALVLMQLAWGWRTMF
VIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPVKPRPQLQFSEWLALFKHRTTWG
MILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIPFLAAAVGMWVNGIVVDRLA
KKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVAFISMALFCVHFAGTSAWGLVQ
VMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTTHSFNLALVIAACVTFTGALCYFF
IVKDRIE