Protein Info for ECOLIN_RS22365 in Escherichia coli Nissle 1917

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 449 transmembrane" amino acids 21 to 51 (31 residues), see Phobius details amino acids 57 to 81 (25 residues), see Phobius details amino acids 93 to 111 (19 residues), see Phobius details amino acids 117 to 138 (22 residues), see Phobius details amino acids 163 to 185 (23 residues), see Phobius details amino acids 197 to 215 (19 residues), see Phobius details amino acids 250 to 274 (25 residues), see Phobius details amino acids 286 to 306 (21 residues), see Phobius details amino acids 315 to 334 (20 residues), see Phobius details amino acids 340 to 360 (21 residues), see Phobius details amino acids 380 to 402 (23 residues), see Phobius details amino acids 408 to 428 (21 residues), see Phobius details PF00083: Sugar_tr" amino acids 22 to 230 (209 residues), 92.9 bits, see alignment E=3.4e-30 amino acids 220 to 427 (208 residues), 40.8 bits, see alignment E=2.1e-14 PF07690: MFS_1" amino acids 28 to 393 (366 residues), 110.5 bits, see alignment E=1.3e-35 PF13347: MFS_2" amino acids 145 to 369 (225 residues), 37.1 bits, see alignment E=2.2e-13

Best Hits

Swiss-Prot: 41% identical to SHIA_ECOLI: Shikimate transporter (shiA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 99% identity to ecg:E2348C_4184)

MetaCyc: 41% identical to shikimate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-27

Predicted SEED Role

"Shikimate transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (449 amino acids)

>ECOLIN_RS22365 MFS transporter (Escherichia coli Nissle 1917)
MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGLVFAPLFYPDSDPFIGL
IASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVGCSTFLIGFIPTYQEIGIW
APLILMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLGSLPQTAASVGIMLATGIFAL
CNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEETLDFQKQKTTNNKEKSVPPLIE
LFKKHPRNILLALGARLAESVSSNIINAFGIVYISSQLALSRDIPLTGMLIASAIGIFSC
PLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAV
QPTLFTRMFGTKVRYTGLSFAYQFSAILGGLSPLIASSLLALGGGKPWYVALFLFAVSVL
SFVCVWLIEPTDEQETASYRYIREQSHEN