Protein Info for ECOLIN_RS17215 in Escherichia coli Nissle 1917

Annotation: inorganic phosphate transporter PitB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 transmembrane" amino acids 6 to 28 (23 residues), see Phobius details amino acids 52 to 77 (26 residues), see Phobius details amino acids 93 to 115 (23 residues), see Phobius details amino acids 123 to 143 (21 residues), see Phobius details amino acids 155 to 177 (23 residues), see Phobius details amino acids 208 to 227 (20 residues), see Phobius details amino acids 233 to 254 (22 residues), see Phobius details amino acids 381 to 402 (22 residues), see Phobius details amino acids 421 to 442 (22 residues), see Phobius details amino acids 449 to 450 (2 residues), see Phobius details amino acids 473 to 495 (23 residues), see Phobius details PF01384: PHO4" amino acids 30 to 488 (459 residues), 318.6 bits, see alignment E=2.4e-99

Best Hits

Swiss-Prot: 99% identical to PITB_ECOLI: Probable low-affinity inorganic phosphate transporter 2 (pitB) from Escherichia coli (strain K12)

KEGG orthology group: K03306, inorganic phosphate transporter, PiT family (inferred from 99% identity to eco:b2987)

MetaCyc: 99% identical to metal phosphate:H+ symporter PitB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-114; TRANS-RXN-277; TRANS-RXN-278; TRANS-RXN0-550

Predicted SEED Role

"Low-affinity inorganic phosphate transporter" in subsystem Phosphate metabolism

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (499 amino acids)

>ECOLIN_RS17215 inorganic phosphate transporter PitB (Escherichia coli Nissle 1917)
MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQLAVVMAAFF
NFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLLAAIIWNLGTWFFGLPASS
SHTLIGAIIGIGLTNALLNGSSVMDALNLREVTKIFSSLIVSPIVGLVIAGGLIFLLRRF
WSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGANDGQKGIGLVMLV
LVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQKLITMEPPLPATSTDGTQ
VTEFHCHPANTFDAIARVKTMLPGNMESYEPLSVSQRSQLRRIMLCISDTSAKLAKLPGV
SKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTMIGWRRVAMTIGEKIGKRGMTY
AQGMAAQMTAAVSIGLASYIGMPVSTTHVLSSAVAGTMVVDGGGLQRKTVTSILMAWVFT
LPAAIFLSGGLYWIALQLI