Protein Info for ECOLIN_RS16015 in Escherichia coli Nissle 1917

Annotation: D-phenylhydantoinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02033: dihydropyrimidinase
  • amino acids 3 to 453 (451 residues), 667.3 bits, E=6e-205
• PF01979: Amidohydro_1
  • amino acids 50 to 434 (385 residues), 69.5 bits, E=3.2e-23

Best Hits

Swiss-Prot: 100% identical to PHYDA_ECOL5: D-phenylhydantoinase (hyuA) from Escherichia coli O6:K15:H31 (strain 536 / UPEC)

KEGG orthology group: K01464, dihydropyrimidinase [EC: 3.5.2.2] (inferred from 100% identity to ecv:APECO1_3653)

MetaCyc: 98% identical to phenylhydantoinase (Escherichia coli K-12 substr. MG1655)
3.5.2.-

Predicted SEED Role

No annotation

MetaCyc Pathways

• superpathway of pyrimidine ribonucleosides degradation (4/5 steps found)
• thymine degradation (2/3 steps found)
• uracil degradation I (reductive) (2/3 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pantothenate and CoA biosynthesis
• Pyrimidine metabolism
• beta-Alanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.2

Protein Sequence (461 amino acids)

>ECOLIN_RS16015 D-phenylhydantoinase (Escherichia coli Nissle 1917)
MRVLIKNGIVVNADGQAKQDLLIESGIVRQLGTDISPQLPCEEIDASGCYVFPGGVDVHT
HFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVI
DYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKLNDDEVLQALRRLHESGALTT
VHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLYIVHL
SNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWC
GISDGAIDVVATDHCTFSMAQRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRISPE
RFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDPRQSQQIQHRHLHDNADYSPWEGFT
CQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL