Protein Info for ECOLIN_RS15950 in Escherichia coli Nissle 1917

Annotation: 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01832: 2-deoxy-D-gluconate 3-dehydrogenase
  • amino acids 6 to 253 (248 residues), 454.2 bits, E=5.7e-141
• PF00106: adh_short
  • amino acids 11 to 203 (193 residues), 193.1 bits, E=5.4e-61
• PF08659: KR
  • amino acids 14 to 163 (150 residues), 23.5 bits, E=7.2e-09
• PF13561: adh_short_C2
  • amino acids 20 to 250 (231 residues), 184.1 bits, E=5e-58

Best Hits

Swiss-Prot: 98% identical to KDUD_ECOLI: 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase (kduD) from Escherichia coli (strain K12)

KEGG orthology group: K00065, 2-deoxy-D-gluconate 3-dehydrogenase [EC: 1.1.1.125] (inferred from 100% identity to ecc:c3439)

MetaCyc: 98% identical to putative 2-keto-3-deoxy-D-gluconate dehydrogenase (Escherichia coli K-12 substr. MG1655)
2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase. [EC: 1.1.1.127]; 1.1.1.- [EC: 1.1.1.127]; RXN0-7101 [EC: 1.1.1.127]

Predicted SEED Role

"2-deoxy-D-gluconate 3-dehydrogenase (EC 1.1.1.125)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 1.1.1.125)

MetaCyc Pathways

• 4-deoxy-L-threo-hex-4-enopyranuronate degradation (5/5 steps found)
• 2-deoxy-D-glucose 6-phosphate degradation (3/4 steps found)
• 3,6-anhydro-α-L-galactopyranose degradation (4/7 steps found)
• superpathway of microbial D-galacturonate and D-glucuronate degradation (20/31 steps found)

KEGG Metabolic Maps

• Pentose and glucuronate interconversions

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.125

Use Curated BLAST to search for 1.1.1.125 or 1.1.1.127

Protein Sequence (253 amino acids)

>ECOLIN_RS15950 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD (Escherichia coli Nissle 1917)
MILNAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVEPTETIKQVTAQGRRFL
SLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDALEFSETDWDDVMNLNIKSV
FFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTASKSGVMGVTRLMANEWAKHN
INVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRWGLPSDLMGPVVFLASSASDYVN
GYTIAVDGGWLAR