Protein Info for ECOLIN_RS14625 in Escherichia coli Nissle 1917

Annotation: protein lysine acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 886 PF13380: CoA_binding_2" amino acids 13 to 130 (118 residues), 81.9 bits, see alignment E=1.2e-26 PF13607: Succ_CoA_lig" amino acids 147 to 280 (134 residues), 154.5 bits, see alignment E=3.7e-49 PF13549: ATP-grasp_5" amino acids 473 to 693 (221 residues), 249.9 bits, see alignment E=4.8e-78 PF13302: Acetyltransf_3" amino acids 725 to 864 (140 residues), 37.2 bits, see alignment E=1.2e-12 PF00583: Acetyltransf_1" amino acids 758 to 863 (106 residues), 47.1 bits, see alignment E=6.9e-16

Best Hits

Swiss-Prot: 99% identical to LYSAC_ECOLI: Peptidyl-lysine N-acetyltransferase PatZ (patZ) from Escherichia coli (strain K12)

KEGG orthology group: K09181, hypothetical protein (inferred from 100% identity to ecc:c3109)

MetaCyc: 99% identical to peptidyl-lysine N-acetyltransferase (Escherichia coli K-12 substr. MG1655)
2.3.1.-

Predicted SEED Role

"Protein acetyltransferase" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (886 amino acids)

>ECOLIN_RS14625 protein lysine acetyltransferase (Escherichia coli Nissle 1917)
MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPVLPVTPAWKAVLGVLAW
PGIARLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSAPASQHEDLRACALRHNMR
LLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVSNTILDWAQQREMGFSYFIAL
GDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFVSAARSASRNKPILVIKSGRSPA
AQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSAVETLSHMRPLRGDRLMIISNGAAP
AALALDALWSRNGKLATLSEETCQKLRDALPGHVAISNPLDLRDDASSEHYVKTLDILLH
SQDFDALMVIHSPSAAAPATESAQVLIEAVKHHPRSKYVSLLTNWCGEHSSQEARRLFSE
AGLPTYRTPEGTITAFMHMVEYRRNQKQLRETPALPSNLTSNTAEAHLLLQHAIAEGATS
LDTHEVQPILQAYGMNTLPTWIASDSTEAVHIAKQIGYPVALKLRSPDIPHKSEVQGVML
YLRTANEVQQAANAIFDRVKMAWPQARVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIM
LGEGGVEWRPEDQAVVALPPLNMNLARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQV
SNLIVDCPEIQRLDIHPLLASGSEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVEL
KNGERCLFRPILPEDEPQLQQFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMA
FVAVRRIDQTEEILGVTRAISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGL
QRLNGITMPNNRGMVALARKLGFNVDIQLEEGIVGLTLNLAQREES