Protein Info for ECOLIN_RS14175 in Escherichia coli Nissle 1917

Annotation: DnaA inactivator Hda

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 233 TIGR03420: DnaA regulatory inactivator Hda" amino acids 6 to 232 (227 residues), 281.5 bits, see alignment E=2.3e-88 PF00308: Bac_DnaA" amino acids 16 to 211 (196 residues), 61 bits, see alignment E=7.9e-21

Best Hits

Swiss-Prot: 100% identical to HDA_ECO8A: DnaA regulatory inactivator Hda (hda) from Escherichia coli O8 (strain IAI1)

KEGG orthology group: K10763, DnaA-homolog protein (inferred from 100% identity to eco:b2496)

Predicted SEED Role

"Chromosomal replication initiator protein DnaA" in subsystem DNA-replication

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (233 amino acids)

>ECOLIN_RS14175 DnaA inactivator Hda (Escherichia coli Nissle 1917)
MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSGYIYLWAREGAGRSHLL
HAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCIDNIECIAGDELWEMAIFDL
YNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARL
RGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIPFVKEILKL