Protein Info for ECOLIN_RS13620 in Escherichia coli Nissle 1917
Annotation: D-serine ammonia-lyase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to SDHD_ECOL6: D-serine dehydratase (dsdA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: K01753, D-serine dehydratase [EC: 4.3.1.18] (inferred from 100% identity to ecc:c2901)MetaCyc: 99% identical to D-serine ammonia-lyase (Escherichia coli K-12 substr. MG1655)
D-serine ammonia-lyase. [EC: 4.3.1.18]
Predicted SEED Role
"D-serine dehydratase (EC 4.3.1.18)" in subsystem Glycine and Serine Utilization or Pyruvate Alanine Serine Interconversions (EC 4.3.1.18)
MetaCyc Pathways
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
- D-serine degradation (3/3 steps found)
- L-serine degradation (3/3 steps found)
- L-tryptophan degradation II (via pyruvate) (3/3 steps found)
- L-methionine biosynthesis II (5/6 steps found)
- L-cysteine degradation II (2/3 steps found)
- purine nucleobases degradation II (anaerobic) (17/24 steps found)
- glycine betaine degradation III (4/7 steps found)
- felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
- L-mimosine degradation (4/8 steps found)
- glycine betaine degradation I (4/8 steps found)
- glutathione-mediated detoxification I (3/8 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.3.1.18
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (442 amino acids)
>ECOLIN_RS13620 D-serine ammonia-lyase (Escherichia coli Nissle 1917) MENAKMNSLIAQYPLVEDLVALKETTWFNPGTTSLAEGLPYVGLTEQDVQDAHARLSRFA PYLAKAFPETAAAGGIIESELVAIPAMQKRLEKEYQQPIAGQLLLKKDSHLPISGSIKAR GGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQYSIAVGSTGNLGLSIGIMSA RIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGVAVEEGRKAAQSDPNCFFIDDEN SRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYLPCGVGGGPGGVAFGLKLAFGDHVH CFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNLTAADGLAVGRASGFVGRAMERLLDGF YTLSDQTMYDMLGWLAQEEGIRLEPSALAGMAGSQRVCASVSYQQMHGFSAEQLRNATHL VWATGGGMVPEEEMNQYLAKGR