Protein Info for ECOLIN_RS13205 in Escherichia coli Nissle 1917

Annotation: ribonuclease BN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 305 TIGR02649: ribonuclease BN" amino acids 2 to 304 (303 residues), 670.6 bits, see alignment E=2.3e-206 TIGR02651: ribonuclease Z" amino acids 2 to 304 (303 residues), 344.4 bits, see alignment E=5.1e-107 PF00753: Lactamase_B" amino acids 30 to 129 (100 residues), 30.8 bits, see alignment E=2.9e-11 PF12706: Lactamase_B_2" amino acids 36 to 154 (119 residues), 36.3 bits, see alignment E=4.6e-13 amino acids 203 to 270 (68 residues), 29.3 bits, see alignment E=6.4e-11

Best Hits

Swiss-Prot: 100% identical to RBN_ECO45: Ribonuclease BN (rbn) from Escherichia coli O45:K1 (strain S88 / ExPEC)

KEGG orthology group: K00784, ribonuclease Z [EC: 3.1.26.11] (inferred from 99% identity to ecg:E2348C_2413)

MetaCyc: 96% identical to ribonuclease BN (Escherichia coli K-12 substr. MG1655)
Ribonuclease Z. [EC: 3.1.26.11]; Exoribonuclease II. [EC: 3.1.26.11, 3.1.13.1]; 3.1.13.1 [EC: 3.1.26.11, 3.1.13.1]

Predicted SEED Role

"Ribonuclease Z (EC 3.1.26.11)" in subsystem tRNA processing (EC 3.1.26.11)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 3.1.13.1 or 3.1.26.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (305 amino acids)

>ECOLIN_RS13205 ribonuclease BN (Escherichia coli Nissle 1917)
MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQHQLLHTAFNPGKLDKI
FISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVETALRISGSWTDYPLEIVE
IGAGEIFDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALNAQALKAAGVPPGPLFQELKA
GKTIMLDDGRQINGADYLAVPVPGKALAIFGDTGPCDAALELAKGVDVMVHEATLDMAME
AKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYDDKGCQHLLRECRSIFPATELANDF
AVFSI