Protein Info for ECOLIN_RS12200 in Escherichia coli Nissle 1917

Annotation: multidrug efflux RND transporter permease subunit MdtB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1040 transmembrane" amino acids 21 to 41 (21 residues), see Phobius details amino acids 345 to 362 (18 residues), see Phobius details amino acids 369 to 390 (22 residues), see Phobius details amino acids 397 to 419 (23 residues), see Phobius details amino acids 440 to 460 (21 residues), see Phobius details amino acids 472 to 496 (25 residues), see Phobius details amino acids 537 to 557 (21 residues), see Phobius details amino acids 863 to 885 (23 residues), see Phobius details amino acids 892 to 912 (21 residues), see Phobius details amino acids 918 to 944 (27 residues), see Phobius details amino acids 965 to 984 (20 residues), see Phobius details amino acids 996 to 1022 (27 residues), see Phobius details PF00873: ACR_tran" amino acids 13 to 1023 (1011 residues), 1255.2 bits, see alignment E=0 PF02355: SecD_SecF" amino acids 348 to 492 (145 residues), 21.1 bits, see alignment E=1.9e-08

Best Hits

Swiss-Prot: 100% identical to MDTB_ECO45: Multidrug resistance protein MdtB (mdtB) from Escherichia coli O45:K1 (strain S88 / ExPEC)

KEGG orthology group: K07788, RND superfamily, multidrug transport protein MdtB (inferred from 66% identity to axy:AXYL_00555)

MetaCyc: 100% identical to multidrug efflux pump RND permease subunit MdtB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-352; TRANS-RXN-353; TRANS-RXN-92

Predicted SEED Role

"Multidrug transporter MdtB"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1040 amino acids)

>ECOLIN_RS12200 multidrug efflux RND transporter permease subunit MdtB (Escherichia coli Nissle 1917)
MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVSALPEVDYPTIQVVTLY
PGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITLQFQLTLPLDVAEQEVQAA
INAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPMTQVEDMVETRVAQKISQISG
VGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAITGANVNSAKGSLDGPSRAVTLSA
NDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAENSWLGAWANKEQAIVMNVQRQPGA
NIISTADSIRQMLPQLTESLPKSVKVTVLSDRTTNIRASVDDTQFELMMAIALVVMIIYL
FLRNIPATIIPGVAVPLSLIGTFAVMVFLDFSINNLTLMALTIATGFVVDDAIVVIENIS
RYIEKGEKPLAAALKGAGEIGFTIISLTFSLIAVLIPLLFMGDIVGRLFREFAITLAVAI
LISAVVSLTLTPMMCARMLSQESLRKQNRFSRASEKMFDRIIAAYGRGLAKVLNHPWLTL
SVALSTLLLSVLLWVFIPKGFFPVQDNGIIQGTLQAPQSSSFANMAQRQRQVADVILQDP
AVQSLTSFVGVDGTNPSLNSARLQINLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQP
TQDLTIDTQVSRTQYQFTLQATSLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYV
NVDRDSASRLGISMADVDNALYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTI
RLTSSDGGVVPLSSIAKIEQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEK
TLNLPVDITTQFQGSTLAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPT
AGVGALLALMIAGSELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQA
CLLRFRPILMTTLAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYL
LFDRLALWTKSRFARHEEEA