Protein Info for ECOLIN_RS11630 in Escherichia coli Nissle 1917
Annotation: bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 98% identical to COBU_ECO57: Bifunctional adenosylcobalamin biosynthesis protein CobU (cobU) from Escherichia coli O157:H7
KEGG orthology group: K02231, adenosylcobinamide kinase / adenosylcobinamide-phosphate guanylyltransferase [EC: 2.7.1.156 2.7.7.62] (inferred from 98% identity to eco:b1993)MetaCyc: 82% identical to adenosylcobinamide-phosphate guanylyltransferase subunit (Salmonella enterica enterica serovar Typhimurium)
Adenosylcobinamide-phosphate guanylyltransferase. [EC: 2.7.7.62]; Adenosylcobinamide kinase. [EC: 2.7.7.62, 2.7.1.156]; 2.7.1.156 [EC: 2.7.7.62, 2.7.1.156]; 2.7.1.156 [EC: 2.7.7.62, 2.7.1.156]
Predicted SEED Role
No annotation
MetaCyc Pathways
- superpathway of adenosylcobalamin salvage from cobinamide I (8/8 steps found)
- adenosylcobinamide-GDP salvage from cobinamide I (5/5 steps found)
- superpathway of adenosylcobalamin salvage from cobinamide II (7/9 steps found)
- adenosylcobinamide-GDP biosynthesis from cobyrinate a,c-diamide (4/6 steps found)
- adenosylcobinamide-GDP salvage from cobinamide II (4/6 steps found)
- adenosylcobinamide-GDP salvage from assorted adenosylcobamides (1/2 steps found)
- adenosylcobalamin biosynthesis II (aerobic) (17/33 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (16/36 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.156
Use Curated BLAST to search for 2.7.1.156 or 2.7.7.62
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (181 amino acids)
>ECOLIN_RS11630 bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (Escherichia coli Nissle 1917) MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVER WQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLI AACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKI K