Protein Info for ECOLIN_RS08895 in Escherichia coli Nissle 1917

Annotation: voltage-gated ClC-type chloride channel ClcB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 transmembrane" amino acids 17 to 45 (29 residues), see Phobius details amino acids 66 to 83 (18 residues), see Phobius details amino acids 112 to 127 (16 residues), see Phobius details amino acids 133 to 151 (19 residues), see Phobius details amino acids 158 to 178 (21 residues), see Phobius details amino acids 180 to 182 (3 residues), see Phobius details amino acids 186 to 212 (27 residues), see Phobius details amino acids 233 to 254 (22 residues), see Phobius details amino acids 270 to 292 (23 residues), see Phobius details amino acids 303 to 325 (23 residues), see Phobius details amino acids 331 to 352 (22 residues), see Phobius details amino acids 362 to 384 (23 residues), see Phobius details amino acids 396 to 416 (21 residues), see Phobius details PF00654: Voltage_CLC" amino acids 72 to 414 (343 residues), 271.3 bits, see alignment E=6.8e-85

Best Hits

Swiss-Prot: 100% identical to CLCB_ECO81: Voltage-gated ClC-type chloride channel ClcB (clcB) from Escherichia coli O81 (strain ED1a)

KEGG orthology group: K03281, chloride channel protein, CIC family (inferred from 100% identity to ecp:ECP_1536)

MetaCyc: 99% identical to putative chloride:H+ antiporter ClcB (Escherichia coli K-12 substr. MG1655)
RXN0-2501

Predicted SEED Role

"FIG00509904: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (430 amino acids)

>ECOLIN_RS08895 voltage-gated ClC-type chloride channel ClcB (Escherichia coli Nissle 1917)
MHHLHIYPDLRTMFRRLLIATIVGILAAFAVAGFRHAMLLLEWLFLNNDSGSLVNAATNL
SPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQTDGQFDYAASLVKSLASL
LVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWIACGAAAGMAAAYRAPLAGSL
FIAEVLFGTMMLASLGPVIISAVVALLISNLINHSDALLYSVQLSVTVQARDYALIISTG
VLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGGLIVGLLSLFTPAVWGNGYSTVQSF
LTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVFTPTLFIGLAIGMLYGRSLGLWLPDGE
EITLLLGLTGMATLLAATTHAPIMSTLMICEMTGEYQLLPGLLIACVIASVISRTLHRDS
IYRQHTAKHS