Protein Info for ECOLIN_RS08665 in Escherichia coli Nissle 1917

Annotation: type II toxin-antitoxin system serine/threonine protein kinase toxin HipA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 439 TIGR03071: HipA N-terminal domain" amino acids 4 to 103 (100 residues), 102.7 bits, see alignment E=5.2e-34 PF13657: Couple_hipA" amino acids 6 to 103 (98 residues), 97.6 bits, see alignment E=5.5e-32 PF07804: HipA_C" amino acids 149 to 402 (254 residues), 179.6 bits, see alignment E=7.1e-57

Best Hits

Swiss-Prot: 98% identical to HIPA_ECOLI: Serine/threonine-protein kinase toxin HipA (hipA) from Escherichia coli (strain K12)

KEGG orthology group: K07154, (no description) (inferred from 99% identity to ecv:APECO1_637)

MetaCyc: 98% identical to serine/threonine-protein kinase toxin HipA (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"HipA protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (439 amino acids)

>ECOLIN_RS08665 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA (Escherichia coli Nissle 1917)
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNF
FDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKL
TEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTALLRIGNDWCIPKGITPTTHII
KLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQV
FQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASK
GKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIPAALDNVKNSLPTDFPENVVT
AVETNVLRLHGRLSPRIRY