Protein Info for ECOLIN_RS08165 in Escherichia coli Nissle 1917

Annotation: bifunctional alpha/beta hydrolase/class I SAM-dependent methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 585 PF12146: Hydrolase_4" amino acids 32 to 266 (235 residues), 223.5 bits, see alignment E=4.9e-70 PF00561: Abhydrolase_1" amino acids 37 to 140 (104 residues), 31.2 bits, see alignment E=3.5e-11 PF12697: Abhydrolase_6" amino acids 54 to 257 (204 residues), 36.4 bits, see alignment E=1.9e-12 PF12147: Methyltransf_20" amino acids 275 to 582 (308 residues), 501.4 bits, see alignment E=1.9e-154

Best Hits

Swiss-Prot: 95% identical to YNBC_ECOLI: Uncharacterized protein YnbC (ynbC) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ecp:ECP_1415)

Predicted SEED Role

"Putative lipase in cluster with Phosphatidate cytidylyltransferase" in subsystem Triacylglycerol metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (585 amino acids)

>ECOLIN_RS08165 bifunctional alpha/beta hydrolase/class I SAM-dependent methyltransferase (Escherichia coli Nissle 1917)
MENSRIPGEHFFTTSDNTALFYRHWPTLLPGAKKVIVLFHRGHEHSGRLQHIVDELAMPD
TAFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDSQVGLEEVVVIAQSVGAVM
VATWVHDYAPAIRGLVLASPAFKVKLYVPLARPGLALWHRLRGLFFINSYVKGRYLTHDR
QRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAITLPTQLLISGDDYVVHRQPQID
FYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQSFISRLYANKSQKFDYQHEDRTGP
SADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGAHSAGLHLGMSTGFDSGSSLDYVYQNQ
PQGSNAFGRFIDKIYLNSVGWRGIRQRKTHLQMLIKQAVAHLHAKGLAVRVVDIAAGHGR
YVLDALANEPAISEILLRDYSEVNVAQGQEMIAQRGMSGRVRFEQGDAFNPEELSALTPR
PTLAIVSGLYELFPENEQVKNSLAGLANAIDPGGILIYTGQPWHPQLELIAGVLTSHKDG
KPWVMRVRSQGEMDSLVHDAGFDKCTQRIDVWGIFTVSMAVRRDN