Protein Info for ECOLIN_RS05025 in Escherichia coli Nissle 1917

Annotation: porin OmpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF13505: OMP_b-brl" amino acids 8 to 195 (188 residues), 82.8 bits, see alignment E=5.5e-27 PF01389: OmpA_membrane" amino acids 23 to 199 (177 residues), 266.3 bits, see alignment E=1.9e-83 PF00691: OmpA" amino acids 226 to 321 (96 residues), 85.3 bits, see alignment E=4.6e-28

Best Hits

Swiss-Prot: 98% identical to OMPA_SHIFL: Outer membrane protein A (ompA) from Shigella flexneri

KEGG orthology group: K03286, OmpA-OmpF porin, OOP family (inferred from 100% identity to ect:ECIAI39_2190)

Predicted SEED Role

"Outer membrane protein A precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (350 amino acids)

>ECOLIN_RS05025 porin OmpA (Escherichia coli Nissle 1917)
MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGFIPNNGPTHENQLGAGA
FGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTAKLGYPITDDLDIYTRLGG
MVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEIATRLEYQWTNNIGDAHTIGT
RPDNGMLSLGVSYRFGQGEVAPVVAPAPAPAPEVQTKHFTLKSDVLFTFNKATLKPEGQA
ALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQALSERRAQSVVDYLISKGIPADKIS
ARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEVKGIKDVVTQPQA