Protein Info for ECOLIN_RS04120 in Escherichia coli Nissle 1917

Annotation: RNA-guided endonuclease TnpB family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 376 PF12323: HTH_OrfB_IS605" amino acids 1 to 45 (45 residues), 75.9 bits, see alignment 2e-25 PF01385: OrfB_IS605" amino acids 172 to 274 (103 residues), 90.4 bits, see alignment E=1.6e-29 TIGR01766: transposase, IS605 OrfB family" amino acids 243 to 314 (72 residues), 36.7 bits, see alignment E=2.3e-13 PF07282: OrfB_Zn_ribbon" amino acids 287 to 355 (69 residues), 81.6 bits, see alignment E=4.5e-27

Best Hits

Swiss-Prot: 54% identical to YSNA_STRPR: Putative transposase in snaA-snaB intergenic region from Streptomyces pristinaespiralis

KEGG orthology group: K07496, putative transposase (inferred from 100% identity to ecp:ECP_0787)

Predicted SEED Role

"Peyer's patch-specific virulence factor GipA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (376 amino acids)

>ECOLIN_RS04120 RNA-guided endonuclease TnpB family protein (Escherichia coli Nissle 1917)
MKRAYKYRFYPTTEQAELLAQTFGCVRFVYNSILRWRTDAYYERKEKIGYLQANARLTAL
KKEPEYIWLNDVSCVPLQQSLRHQQAAFANFFAGRAAYPAFKSKRHKQVAEFTASAFKHR
DGELYIAKSKSPLDVRWSRELPSAPSTVTISRDSAGRYFVSCLCEFEPVSMPVTAKTVGI
DVGLKDLFVTDTGFKTDNPRHTAKYAKRLTLLQRRLSRKQKGSRNRIKARLKVARLHAKI
ADCRMDNLHKLSRKLINENQVVCVESLKVKNMIRNPKLSKAIADAGWSELVRQLQYKGKW
AGRSVVAIDQYLPSSKCCSCCGFTMQKMPLNVRKWHCPECGADHDRDINAARNIKAAGLA
VLAHGEPVNPESQHAA